Any feedback?
Literature summary for 2.7.2.3 extracted from
- Purification and properties of phosphoglycerate kinase from Thermus thermophilus strain HB8 (1979), J. Biochem., 85, 1509-1517.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization from ammonium sulfate precipitation overnight with gradually raising of temperature from 4°C to room temperature, in presence of ATP | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | strong | Thermus thermophilus |  |
| 2,3-diphosphoglycerate | - |
Thermus thermophilus | |
| ADP | - |
Thermus thermophilus | |
| CTP | competitive to ATP | Thermus thermophilus | |
| GTP | competitive to ATP | Thermus thermophilus | |
| ITP | weak competitive inhibitor | Thermus thermophilus | |
| NaCl | weak | Thermus thermophilus | |
| UTP | weak | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
ATP | pH 7.5, 22°C | Thermus thermophilus | |
| 1.79 | - |
3-phospho-D-glycerate | pH 7.5, 22°C | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Thermus thermophilus | |
| Mg2+ | optimum concentration: 8-16 mM | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
1 * 44000, SDS-PAGE | Thermus thermophilus |
| 44600 | - |
sedimentation equilibrium studies | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 259 | - |
purified enzyme | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | high specificity for ATP | Thermus thermophilus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | high specificity for ATP | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + 1,3-diphosphoglycerate | - |
r | |
| CTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus | CDP + 1,3-diphosphoglycerate | - |
? | |
| CTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | CDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus | GDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | low activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | GDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
Thermus thermophilus | IDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | IDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | no activity | Thermus thermophilus | UDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | no activity | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | UDP + 1,3-diphosphoglycerate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE | Thermus thermophilus |
| More | secondary structure: 29% alpha-helix, 11% pleated sheets | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 79 | - |
20 min, no loss of activity | Thermus thermophilus |
| 92 | - |
5 min, 75% loss of activity | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 10 | pH 4.8: about 45% of activity maximum, pH 10.0: about 90% of activity maximum | Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Thermus thermophilus | |
| ATP | required as phosphate donor | Thermus thermophilus | |
| CTP | - |
Thermus thermophilus | |
| GTP | - |
Thermus thermophilus | |
| ITP | - |
Thermus thermophilus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermus thermophilus | analytical electric focusing | - |
5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
