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Literature summary for 2.7.2.3 extracted from
- Phosphoglycerate kinase from animal tissue (1982), Methods Enzymol., 90, 103-110.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Mus musculus |
| bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Homo sapiens |
| bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Oryctolagus cuniculus |
| bovine serum albumin and dithioerythritol stabilize in the enzyme assay | Equus caballus |
| bovine serum albumin and dithioerythritol stabilize in the enzyme assay | trout |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Equus caballus |  |
| 2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Homo sapiens | |
| 2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Mus musculus | |
| 2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | Oryctolagus cuniculus | |
| 2,3-diphosphoglycerate | competitive to 3-phospho-D-glycerate and MgATP2- | trout | |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Equus caballus | |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Homo sapiens | |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Mus musculus | |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Oryctolagus cuniculus | |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
trout | |
| AMP | - |
Equus caballus | |
| AMP | - |
Homo sapiens | |
| AMP | - |
Mus musculus | |
| AMP | - |
Oryctolagus cuniculus | |
| AMP | - |
trout | |
| GMP | - |
Equus caballus | |
| GMP | - |
Homo sapiens | |
| GMP | - |
Mus musculus | |
| GMP | - |
Oryctolagus cuniculus | |
| GMP | - |
trout | |
| IMP | - |
Equus caballus | |
| IMP | - |
Homo sapiens | |
| IMP | - |
Mus musculus | |
| IMP | - |
Oryctolagus cuniculus | |
| IMP | - |
trout | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Mus musculus | |
| additional information | - |
additional information | kinetics | Homo sapiens | |
| additional information | - |
additional information | kinetics | Oryctolagus cuniculus | |
| additional information | - |
additional information | kinetics | Equus caballus | |
| additional information | - |
additional information | kinetics | trout |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| cytosol | - |
trout | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44520 | - |
amino acid sequence | Equus caballus |
| 44660 | - |
erythrocytes, amino acid sequence | Homo sapiens |
| 46000 | - |
1 * 46000, SDS-PAGE | Homo sapiens |
| 46000 | - |
1 * 46000, SDS-PAGE | Equus caballus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| trout | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 101fold from skeletal muscle, 3780fold from erythrocytes | Homo sapiens |
| 135fold | trout |
| 70fold | Oryctolagus cuniculus |
| 99fold from muscle, 333fold from testis | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
| muscle | - |
Mus musculus | - |
| muscle | - |
Homo sapiens | - |
| muscle | - |
Oryctolagus cuniculus | - |
| muscle | - |
Equus caballus | - |
| muscle | - |
trout | - |
| skeletal muscle | - |
Homo sapiens | - |
| skeletal muscle | - |
Oryctolagus cuniculus | - |
| skeletal muscle | - |
Equus caballus | - |
| testis | - |
Mus musculus | - |
| testis | - |
Homo sapiens | - |
| testis | - |
Oryctolagus cuniculus | - |
| testis | - |
Equus caballus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 600 | - |
purified testis enzyme | Mus musculus |
| 680 | - |
purified erythrocyte enzyme | Homo sapiens |
| 695 | - |
purified enzyme | Oryctolagus cuniculus |
| 700 | - |
- |
Oryctolagus cuniculus |
| 700 | - |
- |
Equus caballus |
| 700 | - |
purified enzyme | trout |
| 710 | - |
purified enzyme | Mus musculus |
| 710 | - |
purified muscle enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| as a precipitate in 3.3 M ammonium sulfate, stable for years | Mus musculus |
| as a precipitate in 3.3 M ammonium sulfate, stable for years | Oryctolagus cuniculus |
| as a precipitate in 3.3 M ammonium sulfate, stable for years | trout |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Mus musculus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Homo sapiens | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Equus caballus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
trout | ADP + 1,3-diphosphoglycerate | - |
r | |
| CTP + 3-phospho-D-glycerate | no activity | Mus musculus | CDP + 1,3-diphosphoglycerate | - |
? | |
| CTP + 3-phospho-D-glycerate | no activity | Homo sapiens | CDP + 1,3-diphosphoglycerate | - |
? | |
| CTP + 3-phospho-D-glycerate | no activity | Oryctolagus cuniculus | CDP + 1,3-diphosphoglycerate | - |
? | |
| CTP + 3-phospho-D-glycerate | no activity | trout | CDP + 1,3-diphosphoglycerate | - |
? | |
| dATP + 3-phospho-D-glycerate | - |
Mus musculus | dADP + 1,3-diphosphoglycerate | - |
? | |
| dATP + 3-phospho-D-glycerate | - |
Equus caballus | dADP + 1,3-diphosphoglycerate | - |
? | |
| dGTP + 3-phospho-D-glycerate | - |
Mus musculus | dGDP + 1,3-diphosphoglycerate | - |
? | |
| dGTP + 3-phospho-D-glycerate | - |
Equus caballus | dGDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | - |
Mus musculus | GDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | - |
Homo sapiens | GDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | GDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | - |
Equus caballus | GDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | - |
trout | GDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
Mus musculus | IDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
Homo sapiens | IDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | IDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
Equus caballus | IDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | - |
trout | IDP + 1,3-diphosphoglycerate | - |
? | |
| additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Mus musculus | ? | - |
? | |
| additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Homo sapiens | ? | - |
? | |
| additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Oryctolagus cuniculus | ? | - |
? | |
| additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | Equus caballus | ? | - |
? | |
| additional information | the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid | trout | ? | - |
? | |
| additional information | nucleotide substrate specificity | Mus musculus | ? | - |
? | |
| additional information | nucleotide substrate specificity | Homo sapiens | ? | - |
? | |
| additional information | nucleotide substrate specificity | Oryctolagus cuniculus | ? | - |
? | |
| additional information | nucleotide substrate specificity | trout | ? | - |
? | |
| additional information | no activity with dTTP | Mus musculus | ? | - |
? | |
| additional information | no activity with dTTP | Homo sapiens | ? | - |
? | |
| additional information | no activity with dTTP | Oryctolagus cuniculus | ? | - |
? | |
| additional information | no activity with dTTP | trout | ? | - |
? | |
| UTP + 3-phospho-D-glycerate | - |
Mus musculus | UDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | - |
Homo sapiens | UDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | UDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | - |
Equus caballus | UDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | - |
trout | UDP + 1,3-diphosphoglycerate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Mus musculus |
| monomer | - |
Oryctolagus cuniculus |
| monomer | - |
trout |
| monomer | 1 * 46000, SDS-PAGE | Homo sapiens |
| monomer | 1 * 46000, SDS-PAGE | Equus caballus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mus musculus |
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Oryctolagus cuniculus |
| 25 | - |
assay at | Equus caballus |
| 25 | - |
assay at | trout |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
trout |
| 6 | 8.5 | - |
Homo sapiens |
| 6 | 9.3 | - |
Oryctolagus cuniculus |
| 6.5 | 8.5 | - |
Mus musculus |
| 7.6 | - |
assay at | Mus musculus |
| 7.6 | - |
assay at | Homo sapiens |
| 7.6 | - |
assay at | Oryctolagus cuniculus |
| 7.6 | - |
assay at | Equus caballus |
| 7.6 | - |
assay at | trout |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Mus musculus | |
| ADP | - |
Homo sapiens | |
| ADP | - |
Oryctolagus cuniculus | |
| ADP | - |
Equus caballus | |
| ADP | - |
trout | |
| ATP | required as phosphate donor | Mus musculus | |
| ATP | required as phosphate donor | Homo sapiens | |
| ATP | required as phosphate donor | Oryctolagus cuniculus | |
| ATP | required as phosphate donor | Equus caballus | |
| ATP | required as phosphate donor | trout | |
| dATP | - |
Mus musculus | |
| dATP | - |
Equus caballus | |
| dGTP | - |
Mus musculus | |
| dGTP | - |
Equus caballus | |
| GTP | - |
Mus musculus | |
| GTP | - |
Homo sapiens | |
| GTP | - |
Oryctolagus cuniculus | |
| GTP | - |
Equus caballus | |
| GTP | - |
trout | |
| ITP | - |
Mus musculus | |
| ITP | - |
Homo sapiens | |
| ITP | - |
Oryctolagus cuniculus | |
| ITP | - |
Equus caballus | |
| ITP | - |
trout | |
| UTP | - |
Mus musculus | |
| UTP | - |
Homo sapiens | |
| UTP | - |
Oryctolagus cuniculus | |
| UTP | - |
Equus caballus | |
| UTP | - |
trout | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Mus musculus | |
| 2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Homo sapiens | |
| 2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Oryctolagus cuniculus | |
| 2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | Equus caballus | |
| 2.3 | - |
2,3-diphosphoglycerate | versus MgATP2- | trout | |
| 3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Mus musculus | |
| 3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Homo sapiens | |
| 3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Oryctolagus cuniculus | |
| 3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | Equus caballus | |
| 3.8 | - |
2,3-diphosphoglycerate | versus 3-phospho-D-glycerate | trout | |
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