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Literature summary for 2.7.2.2 extracted from
- Hyperthermophilic carbamate kinase stability and anabolic in vitro activity at alkaline pH (2018), Appl. Environ. Microbiol., 84, e02250-17 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pyrococcus furiosus |
| expressed in Escherichia coli BL21(DE3) cells | Thermococcus sibiricus |
| expressed in Escherichia coli BL21(DE3) cells | Thermococcus barophilus |
| expressed in Escherichia coli BL21(DE3) cells | Fervidobacterium nodosum |
| expressed in Escherichia coli BL21(DE3) cells | Thermosipho melanesiensis |
| expressed in Escherichia coli BL21(DE3) cells | Enterococcus faecalis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Pyrococcus furiosus |  |
| Mg2+ | 10 mM used in assay conditions | Thermococcus sibiricus | |
| Mg2+ | 10 mM used in assay conditions | Thermococcus barophilus | |
| Mg2+ | 10 mM used in assay conditions | Fervidobacterium nodosum | |
| Mg2+ | 10 mM used in assay conditions | Thermosipho melanesiensis | |
| Mg2+ | 10 mM used in assay conditions | Enterococcus faecalis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + carbamate | Pyrococcus furiosus | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Thermococcus sibiricus | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Thermococcus barophilus | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Fervidobacterium nodosum | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Thermosipho melanesiensis | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Enterococcus faecalis | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Enterococcus faecalis ATCC 700802 | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Pyrococcus furiosus ATCC 43587 | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Fervidobacterium nodosum ATCC 35602 | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Thermosipho melanesiensis DSM 120209 | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Thermococcus sibiricus DSM 12597 | - |
ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | Thermococcus barophilus DSM 11836 | - |
ADP + carbamoyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | P0A2X7 | - |
- |
| Enterococcus faecalis ATCC 700802 | P0A2X7 | - |
- |
| Fervidobacterium nodosum | A7HNY8 | - |
- |
| Fervidobacterium nodosum ATCC 35602 | A7HNY8 | - |
- |
| no activity in Clostridium tetani | - |
- |
- |
| Pyrococcus furiosus | P95474 | - |
- |
| Pyrococcus furiosus ATCC 43587 | P95474 | - |
- |
| Thermococcus barophilus | F0LK57 | - |
- |
| Thermococcus barophilus DSM 11836 | F0LK57 | - |
- |
| Thermococcus sibiricus | C6A5J0 | - |
- |
| Thermococcus sibiricus DSM 12597 | C6A5J0 | - |
- |
| Thermosipho melanesiensis | A6LPA8 | - |
- |
| Thermosipho melanesiensis DSM 120209 | A6LPA8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity column chromatography | Pyrococcus furiosus |
| Ni-affinity column chromatography | Thermococcus sibiricus |
| Ni-affinity column chromatography | Thermococcus barophilus |
| Ni-affinity column chromatography | Fervidobacterium nodosum |
| Ni-affinity column chromatography | Thermosipho melanesiensis |
| Ni-affinity column chromatography | Enterococcus faecalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, 6 months, no loss of activity | Pyrococcus furiosus |
| 4°C, purified enzyme, 6 months, no loss of activity | Thermococcus barophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + carbamate | - |
Pyrococcus furiosus | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Thermococcus sibiricus | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Thermococcus barophilus | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Fervidobacterium nodosum | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Thermosipho melanesiensis | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Enterococcus faecalis | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Enterococcus faecalis ATCC 700802 | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Pyrococcus furiosus ATCC 43587 | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Fervidobacterium nodosum ATCC 35602 | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Thermosipho melanesiensis DSM 120209 | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Thermococcus sibiricus DSM 12597 | ADP + carbamoyl phosphate | - |
r | |
| ATP + carbamate | - |
Thermococcus barophilus DSM 11836 | ADP + carbamoyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * about 40000, SDS-PAGE | Pyrococcus furiosus |
| ? | x * about 45000, SDS-PAGE | Thermococcus sibiricus |
| ? | x * about 35000, SDS-PAGE | Thermococcus barophilus |
| ? | x * about 35000, SDS-PAGE | Fervidobacterium nodosum |
| ? | x * about 35000, SDS-PAGE | Thermosipho melanesiensis |
| ? | x * about 35000, SDS-PAGE | Enterococcus faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Fervidobacterium nodosum |
| 80 | - |
- |
Pyrococcus furiosus |
| 80 | - |
- |
Thermococcus sibiricus |
| 80 | - |
- |
Thermococcus barophilus |
| 80 | - |
- |
Thermosipho melanesiensis |
| 80 | - |
- |
Enterococcus faecalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the enzyme is inactivated after incubation at 40°C for 60 h | Fervidobacterium nodosum |
| 40 | - |
there is no measurable change in catalysis after incubation at 40°C for 60 h | Pyrococcus furiosus |
| 40 | - |
there is no measurable change in catalysis after incubation at 40°C for 60 h | Thermococcus sibiricus |
| 40 | - |
there is no measurable change in catalysis after incubation at 40°C for 60 h | Thermococcus barophilus |
| 40 | - |
there is no measurable change in catalysis after incubation at 40°C for 60 h | Thermosipho melanesiensis |
| 40 | - |
there is no measurable change in catalysis after incubation at 40°C for 60 h | Enterococcus faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.9 | - |
- |
Fervidobacterium nodosum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 11.4 | the enzyme is active over the entire pH range studied (7.4-11.4), with optimum activity at pH 9.9 and above | Pyrococcus furiosus |
| 9.4 | 11.4 | the enzyme shows high activity between pH 9.4 and 11.4 | Thermococcus sibiricus |
| 9.4 | 11.4 | the enzyme shows high activity between pH 9.4 and 11.4 | Thermococcus barophilus |
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Release 2023.1 (January 2023)
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