Cloned (Comment) | Organism |
---|---|
gene arcC, genetic organization in the arc operon, sequence comparisons, recombinant expression in Escherichia coli strain DH5alpha | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus pyogenes | |
L-arginine | slight inhibition | Streptococcus pyogenes | |
L-citrulline | - |
Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
Carbamoyl phosphate | recombinant enzyme, pH 6.5, 37°C | Streptococcus pyogenes | |
0.72 | - |
ADP | recombinant enzyme, pH 6.5, 37°C | Streptococcus pyogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34200 | - |
x * 34200, SDS-PAGE | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + carbamoyl phosphate | Streptococcus pyogenes | - |
ATP + NH3 + CO2 | - |
r | |
ATP + NH3 + CO2 | Streptococcus pyogenes | - |
ADP + carbamoyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | A0A0H3BY05 | serotype M49, strain 591, gene arcC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain DH5alpha by affinity chromatography on StrepTactin resin | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + carbamoyl phosphate | - |
Streptococcus pyogenes | ATP + NH3 + CO2 | - |
r | |
ATP + NH3 + CO2 | - |
Streptococcus pyogenes | ADP + carbamoyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 34200, SDS-PAGE | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
ArcC | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptococcus pyogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Streptococcus pyogenes | |
ATP | - |
Streptococcus pyogenes |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the the arginine deiminase system, overview | Streptococcus pyogenes |