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Literature summary for 2.7.2.18 extracted from
- Characterization of a Rhodobacter sphaeroides primary fatty acid kinase (2021), Arch. Microbiol., 203, 861-864 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) Rosetta-2 cells | Cereibacter sphaeroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
ATP | with acetate as cosubstrate, at pH 5.7 and 37°C | Cereibacter sphaeroides |  |
| 14.2 | - |
valerate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 24.5 | - |
Butyrate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 25.8 | - |
propionate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 53.9 | - |
acetate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 109 | - |
Isobutyrate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 10 mM used in assay conditions. The enzyme exhibits about 25% higher specific activity with manganese as a cofactor compared to magnesium | Cereibacter sphaeroides | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | Q3IYF1 | - |
- |
| Cereibacter sphaeroides ATH 2.4.1 | Q3IYF1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | 100% activity | Cereibacter sphaeroides | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | 100% activity | Cereibacter sphaeroides ATH 2.4.1 | ADP + acetyl phosphate | - |
? | |
| ATP + butyrate | 59.5% activity compared to acetate | Cereibacter sphaeroides | ADP + butyryl phosphate | - |
? | |
| ATP + butyrate | 59.5% activity compared to acetate | Cereibacter sphaeroides ATH 2.4.1 | ADP + butyryl phosphate | - |
? | |
| ATP + heptanoate | 22% activity compared to acetate | Cereibacter sphaeroides | ADP + heptanoyl phosphate | - |
? | |
| ATP + hexanoate | 38% activity compared to acetate | Cereibacter sphaeroides | ADP + hexanoyl phosphate | - |
? | |
| ATP + isobutyrate | 26.7% activity compared to acetate | Cereibacter sphaeroides | ADP + isobutyryl phosphate | - |
? | |
| ATP + isobutyrate | 26.7% activity compared to acetate | Cereibacter sphaeroides ATH 2.4.1 | ADP + isobutyryl phosphate | - |
? | |
| ATP + octanoate | 8.4% activity compared to acetate | Cereibacter sphaeroides | ADP + octanoyl phosphate | - |
? | |
| ATP + propionate | 93.3% activity compared to acetate | Cereibacter sphaeroides | ADP + propionyl phosphate | - |
? | |
| ATP + propionate | 93.3% activity compared to acetate | Cereibacter sphaeroides ATH 2.4.1 | ADP + propionyl phosphate | - |
? | |
| ATP + valerate | 43.3% activity compared to acetate | Cereibacter sphaeroides | ADP + valeryl phosphate | - |
? | |
| additional information | the enzyme shows broad substrate specificity for primary fatty acids of varying lengths (C2-C8) | Cereibacter sphaeroides | ? | - |
- |
|
| additional information | the enzyme shows broad substrate specificity for primary fatty acids of varying lengths (C2-C8) | Cereibacter sphaeroides ATH 2.4.1 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Cereibacter sphaeroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetate kinase | - |
Cereibacter sphaeroides |
| ACK | - |
Cereibacter sphaeroides |
| short chain fatty acid kinase | - |
Cereibacter sphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
activity declines sharply at temperatures above 51°C, with 44% and 12% of activity remaining at 60°C and 67°C, respectively | Cereibacter sphaeroides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 65.4 | - |
Isobutyrate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 80.2 | - |
valerate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 118 | - |
Butyrate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 172 | - |
propionate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 177 | - |
acetate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 250 | - |
ATP | with acetate as cosubstrate, at pH 5.7 and 37°C | Cereibacter sphaeroides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
- |
Cereibacter sphaeroides |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Isobutyrate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 3.3 | - |
acetate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 4.8 | - |
Butyrate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 5.6 | - |
valerate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 6.7 | - |
propionate | at pH 5.7 and 37°C | Cereibacter sphaeroides | |
| 80.5 | - |
ATP | with acetate as cosubstrate, at pH 5.7 and 37°C | Cereibacter sphaeroides | |
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Release 2023.1 (January 2023)
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