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Literature summary for 2.7.2.16 extracted from
- Biosynthesis of cyclic 2,3-diphosphoglycerate. Isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from Methanothermus fervidus (1990), FEBS Lett., 272, 94-98 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, almost complete inhibition | Methanothermus fervidus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
ATP | pH 7.5, 75°C | Methanothermus fervidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 0.3 M, 1.4fold stimulation | Methanothermus fervidus | |
| Mg2+ | required, 8% residual activity in absence of Mg2+ | Methanothermus fervidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 1400000 | - |
gel filtration | Methanothermus fervidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermus fervidus | Q49156 | - |
- |
| Methanothermus fervidus ATCC 43054 | Q49156 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermus fervidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
pH 7.5, 75°C | Methanothermus fervidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-phospho-D-glycerate | - |
Methanothermus fervidus | ADP + 2,3-diphospho-D-glycerate | - |
? | |
| ATP + 2-phospho-D-glycerate | - |
Methanothermus fervidus ATCC 43054 | ADP + 2,3-diphospho-D-glycerate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 32500, SDS-PAGE | Methanothermus fervidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2PGK | - |
Methanothermus fervidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Methanothermus fervidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 108 | - |
ATP | pH 7.5, 75°C | Methanothermus fervidus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Methanothermus fervidus |
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