Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Butyrate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
CTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
dATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
dCTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
dGTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
propionate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
TTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with ATP, GTP, or CTP, soaking of crystals in 0.1 M Bis-Tris, pH 6.5, 35% pentaerythritol ethoxylate, crystals of enzyme StTdcD in complex with phosphate, AMP, ATP, Ap4, GMP, GDP, GTP, CMP and CTP are obtained using variations of crystallization conditions reported for the unliganded enzyme, X-ray diffraction structure determination and analyis at 2.4-3.0 A resolution, molecular replacement method using a polyalanine model of the unliganded StTdcD protomer, PDB ID 2E1Y as the search model, modeling | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | feedback inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
citrate | feedback inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
malate | feedback inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
succinate | feedback inhibition | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetic analysis, the enzyme shows open and closed states consistent with inter-domain motion during catalysis | Salmonella enterica subsp. enterica serovar Typhimurium | |
2 | 14 | UTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.5 | - |
propanoate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
25.3 | - |
acetate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
35.2 | - |
Butyrate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
115 | - |
GTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
126 | - |
ATP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
226 | - |
CTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | activates, required. Free Mg2+ competes with the catalytically suitable ATP-Mg2+ complex for binding to the enzyme, consistent with the conformational differences observed in the binding of phosphate moiety of a nucleotide in the presence or absence of a metal ion | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | O06961 | gene tdcD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + acetyl phosphate | - |
r | |
ATP + butyrate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + butanoyl phosphate | - |
r | |
ATP + propanoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + propanoyl phosphate | - |
r | |
CTP + propanoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CDP + propanoyl phosphate | - |
r | |
GTP + propanoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | GDP + propanoyl phosphate | - |
r | |
additional information | enzyme binding of nucleotide mainly involves hydrophobic interactions with the base moiety and can account for the broad biochemical specificity observed between the enzyme and nucleotides, modeling. Residue Ala88 is an important residue involved in determining the rate of catalysis with short-chain fatty acid substrates. No activity with with several closely related analogues such as formate, isobutyrate, 2-oxobutyrate, malic acid, citrate, isocitric acid, oxaloacetate, succinate and 2-oxoglutarate, and also no activit with short-chain alcohols methanol, ethanol, propanol, and butanol, and aldehydes acetaldehyde and propionaldehyde. An ethylene glycol molecule bound sufficiently close to the gamma-phosphate in StTdcD complexes with triphosphate nucleotides supports direct in-line phosphoryl transfer | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
UTP + propanoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | UDP + propanoyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | molecular dynamics simulations on monomeric and dimeric forms of enzyme StTdcD, modeling, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
TdcD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
Butyrate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
73 | - |
CTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
81 | - |
UTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
108 | - |
propanoate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
113 | - |
GTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
120 | - |
ATP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
120 | - |
acetate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
CTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | enzyme binding of nucleotide mainly involves hydrophobic interactions with the base moiety and can account for the broad biochemical specificity observed between the enzyme and nucleotides, modeling | Salmonella enterica subsp. enterica serovar Typhimurium | |
UTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
General Information | Comment | Organism |
---|---|---|
physiological function | propionate kinase catalyzes reversible transfer of the gamma-phosphate of ATP to propionate during L-threonine degradation to propionate with broad ligand specificity | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
UTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.31 | - |
CTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1 | - |
ATP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1 | - |
GTP | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.7 | - |
Butyrate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.7 | - |
acetate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
43.2 | - |
propanoate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |