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Literature summary for 2.7.2.15 extracted from

  • Simanshu, D.K.; Savithri, H.S.; Murthy, M.R.
    Crystal structures of Salmonella typhimurium propionate kinase and its complex with Ap(4)A: Evidence for a novel Ap(4)A synthetic activity (2007), Proteins, 70, 1379-1388.
    View publication on PubMed
Search for more literature for 2.7.2.15

Cloned(Commentary)

Cloned (Comment) Organism
cloned in pRSET C vector and overexpressed in Escherichia coli BL21(DE3)pLysS along with an N-terminal hexahistidine tag Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
by the hanging drop method, crystals of native TdcD (2.6 A resolution), TdcD-Ap4A complex in the presence of ATP (2.0 A resolution), and TdcD-Ap4A (2.4 A resolution), Ap4A is bound at the active site pocket, Ap4A is present in an extended conformation with one adenosine moiety present in the nucleotide binding site and other in the propionate binding site Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by Ni-NTA affinity column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TdcD has Ap4A synthetic activity in the presence of ATP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

Synonyms Comment Organism
TdcD
-
 Salmonella enterica subsp. enterica serovar Typhimurium