Cloned (Comment) | Organism |
---|---|
gene CL6EHI_170010, DNA and amino acid sequence determination and analysis, quantitative expression analysis by qRT-PCR, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constructio of an acetate kinase antisense knockout mutant strain | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
acetyl phosphate | pH 7.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
0.07 | - |
acetyl phosphate | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
2.2 | - |
phosphate | pH 7.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
2.6 | - |
phosphate | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
- |
Entamoeba histolytica |
77000 | - |
- |
Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphate + acetyl phosphate | Entamoeba histolytica | - |
diphosphate + acetate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | C4M1C3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amoeba | - |
Entamoeba histolytica | - |
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + acetate | - |
Entamoeba histolytica | phosphate + acetyl phosphate | - |
r | |
phosphate + acetyl phosphate | - |
Entamoeba histolytica | diphosphate + acetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, about, sequence calculation, x * 77000, about, recombinant His6-tagged enzyme, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
ACK | - |
Entamoeba histolytica |
PPi-forming acetate kinase | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
acetyl phosphate | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
30 | - |
acetyl phosphate | pH 7.0, 37°C, recombinant enzyme | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
diphosphate and acetate formation reaction, assay at | Entamoeba histolytica |
7 | - |
acetyl phosphate formation reaction, assay at | Entamoeba histolytica |
General Information | Comment | Organism |
---|---|---|
malfunction | AcK gene silencing does not affect acetate production in the parasites but promotes a slight decrease (10-20%) in the hexose phosphates and phosphate levels. Amoebae lacking ADP-forming acetyl-CoA synthetase activity are unable to reestablish the acetyl-CoA/CoA ratio after an oxidative stress challenge | Entamoeba histolytica |
physiological function | The enzyme does not importantly contribute to ATP and phospate supply in the amoebae. Recombinant enzyme AcK shows activity only in the acetate formation direction. Enzyme AcK does not contribute to acetate production but might be marginally involved in phosphate and hexosephosphate homeostasis | Entamoeba histolytica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
phosphate | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
12 | - |
phosphate | pH 7.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
300 | - |
acetyl phosphate | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
1500 | - |
acetyl phosphate | pH 7.0, 37°C, recombinant enzyme | Entamoeba histolytica |