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Literature summary for 2.7.2.11 extracted from

  • Makafe, G.G.; Hussain, M.; Surineni, G.; Tan, Y.; Wong, N.K.; Julius, M.; Liu, L.; Gift, C.; Jiang, H.; Tang, Y.; Liu, J.; Tan, S.; Yu, Z.; Liu, Z.; Lu, Z.; Fang, C.; Zhou, Y.; Zhang, J.; Zhu, Q.; Liu, J.; Zhang, T.
    Quinoline derivatives kill Mycobacterium tuberculosis by activating glutamate kinase (2019), Cell Chem. Biol., 26, 1187-1194 .
    View publication on PubMed
Search for more literature for 2.7.2.11

Activating Compound

Activating Compound Comment Organism Structure
4-(11H-indolo[3,2-c]quinolin-6-yl)aniline Z0933M Mycobacterium tuberculosis
6-(4-(pyrrolidin-1-yl)phenyl)-11H-pyrido[2',3':4,5]pyrrolo[3,2-c]quinoline Z0930 Mycobacterium tuberculosis
tert-butyl(4-(11H-indolo[3,2-c]quinolin-6-yl)phenyl)carbamate Z0933 Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (lambdaDE3) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
A226S the mutant has 1.2fold increased activity and higher thermostability compared to the wild type enzyme Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
L-proline feedback inhibitor Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
92.8
-
 L-glutamate at pH 7.2 and 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 80 mM used in assay conditions Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate Mycobacterium tuberculosis
-
 ADP + L-glutamate 5-phosphate
-
 ?
ATP + L-glutamate Mycobacterium tuberculosis H37Rv
-
 ADP + L-glutamate 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHU9
-
-
Mycobacterium tuberculosis H37Rv P9WHU9
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate
-
 Mycobacterium tuberculosis ADP + L-glutamate 5-phosphate
-
 ?
ATP + L-glutamate
-
 Mycobacterium tuberculosis H37Rv ADP + L-glutamate 5-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function enzyme activation kills Mycobacterium tuberculosis via induction of proline and reactive oxygen species overproduction Mycobacterium tuberculosis