General Stability | Organism |
---|---|
upon concentration by vacuum dialysis against 20 mM imidazole buffer, pH 7, a loss of about 40% of activity occurs | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
GTP | pH 7, 30°C | Entamoeba histolytica | |
0.3 | - |
GDP | pH 7, 30°C | Entamoeba histolytica | |
0.4 | - |
3-phospho-D-glycerate | pH 7, 30°C | Entamoeba histolytica | |
0.7 | - |
IDP | pH 7, 30°C | Entamoeba histolytica | |
0.8 | - |
ITP | pH 7, 30°C | Entamoeba histolytica | |
1.2 | - |
ATP | pH 7, 30°C | Entamoeba histolytica | |
10 | - |
ADP | pH 7, 30°C | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + 3-phospho-D-glyceroyl 1-phosphate | Entamoeba histolytica | production of GTP | GTP + 3-phospho-D-glycerate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
GDP + 3-phospho-D-glyceroyl phosphate | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
4°C, concentrated enzyme solution, 10% loss of activity per week | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | in glycolytic direction selectivity for GDP over ADP is 150-fold, selectivity for GTP over ATP is about 50fold | Entamoeba histolytica | ADP + 3-phospho-D-glyceroyl 1-phosphate | - |
r | |
GDP + 3-phospho-D-glyceroyl 1-phosphate | production of GTP | Entamoeba histolytica | GTP + 3-phospho-D-glycerate | - |
r | |
GTP + 3-phospho-D-glycerate | in glycolytic direction selectivity for GDP over ADP is 150-fold, selectivity for GTP over ATP is about 50fold | Entamoeba histolytica | GDP + 3-phospho-D-glyceroyl 1-phosphate | - |
r | |
GTP + 3-phospho-D-glycerate | - |
Entamoeba histolytica | GDP + 3-phospho-D-glyceroyl phosphate | - |
? | |
ITP + 3-phospho-D-glycerate | in glycolytic direction 40% of the activity with GDP, 30% of the activity with GTP | Entamoeba histolytica | IDP + 3-phospho-D-glyceroyl 1-phosphate | - |
r |