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Literature summary for 2.7.2.1 extracted from

  • Yoshida, Y.; Sato, M.; Nonaka, T.; Hasegawa, Y.; Kezuka, Y.
    Characterization of the phosphotransacetylase-acetate kinase pathway for ATP production in Porphyromonas gingivalis (2019), J. Oral Microbiol., 11, 1588086 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Porphyromonas gingivalis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M lithium sulfate monohydrate, 0.1 M Tris/HCl (pH 8.5), and 24% (w/v) PEG 3350 Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
E385A inactive Porphyromonas gingivalis
R241A the mutant shows 3.9% of wild type activity Porphyromonas gingivalis
R91A the mutant shows about 0.6% of wild type activity Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ less than 1% residual activity at 1.3 mM Porphyromonas gingivalis
EDTA complete inhibition at 1.3 mM Porphyromonas gingivalis
Zn2+ less than 5% residual activity at 1.3 mM Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0222
-
 acetyl phosphate at pH 8.0 and 37°C Porphyromonas gingivalis
0.188
-
 ADP at pH 8.0 and 37°C Porphyromonas gingivalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.3 mM used in assay conditions. The activity of the enzyme in the presence of Mg2+ is about 80% of that in the presence of Mn2+ Porphyromonas gingivalis
Mn2+ Mn2+ supports highest enzyme activity Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
 gel filtration Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + acetyl phosphate Porphyromonas gingivalis
-
 ATP + acetate
-
 r
ADP + acetyl phosphate Porphyromonas gingivalis ATCC 33277
-
 ATP + acetate
-
 r

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis B2RK02
-
-
Porphyromonas gingivalis ATCC 33277 B2RK02
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography, MonoQ column chromatography and Superdex 200 pg gel filtration Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + acetyl phosphate
-
 Porphyromonas gingivalis ATP + acetate
-
 r
ADP + acetyl phosphate
-
 Porphyromonas gingivalis ATCC 33277 ATP + acetate
-
 r

Subunits

Subunits Comment Organism
homodimer or homotrimer 2 or 3 * 43000, SDS-PAGE Porphyromonas gingivalis
homodimer or homotrimer 2 or 3 * 43200, calculated from amino acid sequence Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
ACK
-
 Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
105
-
 acetyl phosphate at pH 8.0 and 37°C Porphyromonas gingivalis
1487
-
 ADP at pH 8.0 and 37°C Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Porphyromonas gingivalis

pH Range

pH Minimum pH Maximum Comment Organism
6 10 about 45% activity at pH 6.0, about 70% activity at pH 7.0, 100% activity at pH 8.0, about 80% activity at pH 9.0, about 60% activity at pH 10.0 Porphyromonas gingivalis