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Literature summary for 2.7.2.1 extracted from
- Acetate kinase-an enzyme of the postulated phosphoketolase pathway in Methylomicrobium alcaliphilum 20Z (2015), Antonie van Leeuwenhoek, 108, 965-974.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ack, DNA and amino acid sequence determination, RT-PCR analysis, transcriptional organization, phylogenetic analysis in Methylotrophic bacteria, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Methylotuvimicrobium alcaliphilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the dependence of the activity on acetate and acetyl phosphate concentrations obeys the Michaelis-Menten kinetics, whereas the dependence on ATP and ADP concentrations is sigmoidal | Methylotuvimicrobium alcaliphilum | |
| 0.11 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |  |
| 1.28 | - |
ADP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 2.36 | - |
ATP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 5.6 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partly substitute for Mn2+ or Mg2+ | Methylotuvimicrobium alcaliphilum | |
| Mg2+ | dependence on Mg2+ or Mn2+ ions, Km values is 0.28 mM | Methylotuvimicrobium alcaliphilum | |
| Mn2+ | dependence on Mg2+ or Mn2+ ions, Km values is 0.47 mM | Methylotuvimicrobium alcaliphilum | |
| additional information | no activity with Ba2+, Ca2+, Zn2+, or Cu2+ | Methylotuvimicrobium alcaliphilum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
- |
Methylotuvimicrobium alcaliphilum |
| 80000 | - |
recombinant His6-tagged enzyme, native PAGE | Methylotuvimicrobium alcaliphilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Methylotuvimicrobium alcaliphilum | - |
ADP + acetyl phosphate | - |
r | |
| ATP + acetate | Methylotuvimicrobium alcaliphilum 20Z | - |
ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylotuvimicrobium alcaliphilum | G4T0C7 | gene ack | - |
| Methylotuvimicrobium alcaliphilum 20Z | G4T0C7 | gene ack | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Methylotuvimicrobium alcaliphilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Methylotuvimicrobium alcaliphilum | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | the enzyme is 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation and shows strong specificity to ATP/ADP The enzyme has a remarkably high catalytic efficiency for acetate and ATP formation | Methylotuvimicrobium alcaliphilum | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | - |
Methylotuvimicrobium alcaliphilum 20Z | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | the enzyme is 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation and shows strong specificity to ATP/ADP The enzyme has a remarkably high catalytic efficiency for acetate and ATP formation | Methylotuvimicrobium alcaliphilum 20Z | ADP + acetyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, recombinant His6-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACK | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant enzyme | Methylotuvimicrobium alcaliphilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | activity range, profile overview | Methylotuvimicrobium alcaliphilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | purified recombinant enzyme, pH 8.0, 50-70% activity is retained after a 3 h exposure at 30-70°C | Methylotuvimicrobium alcaliphilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.28 | - |
ADP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 2.36 | - |
ATP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 233 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 3167 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | recombinant enzyme | Methylotuvimicrobium alcaliphilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | activity range | Methylotuvimicrobium alcaliphilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | strong specificity to ATP/ADP | Methylotuvimicrobium alcaliphilum | |
| ATP | strong specificity to ATP/ADP | Methylotuvimicrobium alcaliphilum | |
| additional information | no activity with CTP, GTP, or UTP | Methylotuvimicrobium alcaliphilum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the postulated phosphoketolase pathway | Methylotuvimicrobium alcaliphilum |
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