Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
crystal structures of Salmonella typhimurium AckA unliganded (Form-I, 2.70 A resolution) and citrate-bound (Form-II, 1.90 A resolution) are reported. Unexpectedly, Form-II StAckA structure show a drastic change in the conformation of residues 230-300 compared to that of Form-I | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-diaminobutyric acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2-oxobutyrate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2-oxoglutarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
citrate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
succinate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ATP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.075 | - |
ATP | co-substrate: propionate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.078 | - |
GTP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.088 | - |
CTP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.096 | - |
UTP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
ADP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.28 | - |
acetyl phosphate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2 | - |
acetate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
11.2 | - |
propionate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
13.5 | - |
formate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
13.5 | - |
ATP | co-substrate: formate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Vmax: 15%, compared to Mg2+ or Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | Vmax: 100% | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | Vmax: 100% | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ni2+ | Vmax: 5%, compared to Mg2+ or Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
calculated from cDNA | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | P63411 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, 15 days, no loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + acetyl phosphate | - |
r | |
ATP + formate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + formyl phosphate | - |
? | |
ATP + propionate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + propionyl phosphate | - |
? | |
CTP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CDP + acetyl phosphate | - |
? | |
GTP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | GDP + acetyl phosphate | - |
? | |
UTP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | UDP + acetyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
StAckA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 37 | optimal temperature range | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
870 | - |
ATP | co-substrate: formate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
880 | - |
formate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
900 | - |
ATP | co-substrate: propionate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
940 | - |
propionate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1170 | - |
ATP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1180 | - |
acetate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1750 | - |
ADP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1764 | - |
acetyl phosphate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.5 | optimal pH range | Salmonella enterica subsp. enterica serovar Typhimurium |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
3 | - |
pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxobutyrate | |
5 | - |
pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | 2,4-diaminobutyric acid | |
7 | - |
pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoglutarate | |
63 | - |
pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | citrate | |
75 | - |
pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | succinate |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68 | - |
formate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
85 | - |
propionate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
985 | - |
acetate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
6300 | - |
acetyl phosphate | pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
10240 | - |
ATP | co-substrate: formate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
11350 | - |
ATP | co-substrate: propionate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
16860 | - |
ATP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
17650 | - |
ADP | co-substrate: acetate, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |