Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, reporter system used that allows cloning of promoter fragments in front of a promoterless lacZ gene and insertion in Mycoplasma pneumoniae | Mycoplasma pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Mycoplasma pneumoniae | - |
ADP + acetyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma pneumoniae | P75245 | wild-type strain M129 and mutant strains | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Mycoplasma pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
transcription initiation and regulation controlled in a carbon source dependent manner, ackA gene encoding acetate kinase is strongly expressed in the presence of glucose, promoter mapping by primer extension, promoter recognition sites studied by promoter deletion analysis, -35 region seems to be of minor importance | Mycoplasma pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Mycoplasma pneumoniae | ADP + acetyl phosphate | - |
? | |
ATP + acetate | transcriptional control of the ackA gene | Mycoplasma pneumoniae | ADP + acetyl phosphate | - |
? |