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Literature summary for 2.7.2.1 extracted from

  • Mukhopadhyay, S.; Hasson, M.S.; Sanders, D.A.
    A continuous assay of acetate kinase activity: measurement of inorganic phosphate release generated by hydroxylaminolysis of acetyl phosphate (2008), Bioorg. Chem., 36, 65-69.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.1

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine inhibits acetate kinase reaction in a nonlinear and noncompetitive fashion, substantial inhibition at concentrations of 704 mM and minimal inhibition at concentrations of 250 microM hydroxylamine Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information lower Km of the Thermotoga maritima acetate kinase for acetate measured than that determined by earlier assay methods Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Thermotoga maritima
-
 ADP + acetyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYB1
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 continuous assay, spectrophotometric quantification of phosphate achieved through measurement of the phosphorylysis of 2-amino-6-mercapto-7-methyl-purine riboside (MESG) to ribose-1-phosphate and 2-amino-6-mercapto-7-methyl-purine (MES) by purine nucleoside phosphorylase, sensitivity of the assay is in the range of 2-150 microM Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + hydroxylamine high-energy acetyl phosphate reacts with hydroxylamine forming acetyl hydroxamate and inorganic phosphate Thermotoga maritima acetyl hydroxamate + phosphate
-
 ?
ATP + acetate
-
 Thermotoga maritima ADP + acetyl phosphate
-
 ?
ATP + acetate enzyme assay developed that can measure the released phosphate at a nanomolar level Thermotoga maritima ADP + acetyl phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 assay can be performed over pH range of Thermotoga maritima