Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.1 extracted from

  • Ren, N.Q.; Lin, H.L.; Zhang, K.; Zheng, G.X.; Duan, Z.J.; Lin, M.
    Cloning, expression, and characterization of an acetate kinase from a high rate of biohydrogen bacterial strain Ethanoligenens sp. hit B49 (2007), Curr. Microbiol., 55, 167-172.
    View publication on PubMed
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Ethanoligenens harbinense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43220
-
 calculated from amino acid sequence Ethanoligenens harbinense

Organism

Organism UniProt Comment Textmining
Ethanoligenens harbinense Q2N1I6 strain hit B49
-

Purification (Commentary)

Purification (Comment) Organism
using imidazole elution [20 mM Tris-HCl, pH 7.9, 0.5 M NaCl, 10% glycerol, 8 M urea, 1 mM PMSF (pH 7.9)] buffers containing final concentrations of imidazole (100 mM, 200 mM, and 1 M imidazole) Ethanoligenens harbinense

Renatured (Commentary)

Renatured (Comment) Organism
using 1 mol/l urea, 50 mM Tris-HCl, pH 8.0, 10 mM reduced glutathione, 2 mmol GSSG, 1 mM FeCl2, 2 mM dithiothreitol, and 10% glycerol, the renaturation yield is 14.36% Ethanoligenens harbinense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.12
-
 at 30°C Ethanoligenens harbinense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Ethanoligenens harbinense ADP + acetyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ACK
-
 Ethanoligenens harbinense

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Ethanoligenens harbinense

pI Value

Organism Comment pI Value Maximum pI Value
Ethanoligenens harbinense calculated from amino acid sequence
-
 5.93