Crystallization (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
F179A | reduced catalytic efficiency with all substrates tested | Methanosarcina thermophila |
L122A | reduced catalytic efficiency with all substrates tested | Methanosarcina thermophila |
P232A | reduced catalytic efficiency with all substrates tested | Methanosarcina thermophila |
V93A | improved catalytic efficiency with propionate and butyrate | Methanosarcina thermophila |
V93G | improved catalytic efficiency with propionate and butyrate | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0499 | - |
ATP | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
0.0506 | - |
ATP | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
0.0542 | - |
ATP | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
0.0576 | - |
ATP | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
0.0687 | - |
ATP | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
0.0713 | - |
ATP | wild-type, pH 7.0 | Methanosarcina thermophila | |
0.15 | - |
acetyl phosphate | mutant F179A, pH 7.4 | Methanosarcina thermophila | |
0.34 | - |
acetyl phosphate | wild-type, pH 7.4 | Methanosarcina thermophila | |
0.5 | - |
acetyl phosphate | mutant L122A, pH 7.4 | Methanosarcina thermophila | |
0.71 | - |
acetyl phosphate | mutant V93G, pH 7.4 | Methanosarcina thermophila | |
0.74 | - |
acetyl phosphate | mutant V93A, pH 7.4 | Methanosarcina thermophila | |
1.19 | - |
acetyl phosphate | mutant P232A, pH 7.4 | Methanosarcina thermophila | |
1.5 | - |
acetate | wild-type, pH 7.0 | Methanosarcina thermophila | |
6.2 | - |
propionate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
10.4 | - |
acetate | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
10.7 | - |
propionate | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
11 | - |
propionate | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
14.4 | - |
propionate | wild-type, pH 7.0 | Methanosarcina thermophila | |
19.5 | - |
acetate | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
21.4 | - |
acetate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
25 | - |
propionate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
32 | - |
acetate | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
33.4 | - |
Butyrate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
39 | - |
Butyrate | wild-type, pH 7.0 | Methanosarcina thermophila | |
39.5 | - |
acetate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
46 | - |
propionate | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
63 | - |
Butyrate | mutant V93G, pH 7.0 | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + acetate = ADP + acetyl phosphate | residue F179 is essential for catalysis | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + hydroxylamine | - |
Methanosarcina thermophila | acetyl hydroxamate + phosphate | - |
r | |
ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
ATP + butyrate | - |
Methanosarcina thermophila | ADP + butyryl phosphate | - |
r | |
ATP + propionate | - |
Methanosarcina thermophila | ADP + propionyl phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
Butyrate | wild-type, pH 7.0 | Methanosarcina thermophila | |
0.37 | - |
propionate | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
2.2 | - |
ATP | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
6.7 | - |
propionate | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
8.5 | - |
propionate | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
12 | - |
acetyl phosphate | mutant F179A, pH 7.4 | Methanosarcina thermophila | |
42.4 | - |
Butyrate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
132 | - |
ATP | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
150 | - |
ATP | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
218 | - |
propionate | wild-type, pH 7.0 | Methanosarcina thermophila | |
294 | - |
Butyrate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
347 | - |
ATP | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
840 | - |
propionate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
844 | - |
ATP | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
1029 | - |
propionate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
1039 | - |
acetyl phosphate | mutant L122A, pH 7.4 | Methanosarcina thermophila | |
1055 | - |
ATP | wild-type, pH 7.0 | Methanosarcina thermophila | |
2396 | - |
acetyl phosphate | mutant P232A, pH 7.4 | Methanosarcina thermophila | |
2572 | - |
acetyl phosphate | mutant V93G, pH 7.4 | Methanosarcina thermophila | |
2680 | - |
acetyl phosphate | wild-type, pH 7.4 | Methanosarcina thermophila | |
3869 | - |
acetyl phosphate | mutant V93A, pH 7.4 | Methanosarcina thermophila |