Cloned (Comment) | Organism |
---|---|
ack gene identified by PCR, heterologously expressed in Escherichia coli JM 109 | Fructilactobacillus sanfranciscensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ADP | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis | |
0.6 | - |
ATP | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis | |
1 | - |
acetyl phosphate | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis | |
48 | - |
acetate | pH 7.5, 30°C | Fructilactobacillus sanfranciscensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Fructilactobacillus sanfranciscensis | |
Mn2+ | - |
Fructilactobacillus sanfranciscensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Fructilactobacillus sanfranciscensis |
57000 | - |
gel filtration | Fructilactobacillus sanfranciscensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Fructilactobacillus sanfranciscensis | key enzyme and responsible for dephosphorylation of acetyl phosphate with the concomitant production of acetate and ATP | ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fructilactobacillus sanfranciscensis | Q9F1X7 | DSM20451, same nucleotide sequence accession number in EMBL/GenBank | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Fructilactobacillus sanfranciscensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
190 U/mg | Fructilactobacillus sanfranciscensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Fructilactobacillus sanfranciscensis | ADP + acetyl phosphate | - |
r | |
ATP + acetate | key enzyme and responsible for dephosphorylation of acetyl phosphate with the concomitant production of acetate and ATP | Fructilactobacillus sanfranciscensis | ADP + acetyl phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Fructilactobacillus sanfranciscensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
80 | - |
Fructilactobacillus sanfranciscensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | stable for 30 min at 60°C, loses activity after 30 min at 70°C, 10 min at 80°C and 5 min at 90°C | Fructilactobacillus sanfranciscensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Fructilactobacillus sanfranciscensis |