Cloned (Comment) | Organism |
---|---|
wild-type and variant acetate kinases are overproduced in Escherichia coli BL21(DE3) | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
H123A | site-directed mutagenesis | Methanosarcina thermophila |
H152A | site-directed mutagenesis | Methanosarcina thermophila |
H180A | site-directed mutagenesis | Methanosarcina thermophila |
H180R | site-directed mutagenesis | Methanosarcina thermophila |
H184A | site-directed mutagenesis | Methanosarcina thermophila |
H208A | site-directed mutagenesis | Methanosarcina thermophila |
H60A | site-directed mutagenesis | Methanosarcina thermophila |
H90A | site-directed mutagenesis | Methanosarcina thermophila |
H94A | site-directed mutagenesis | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | - |
Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | pH 7.0, 37°C, mutant H180A, produced in E. coli | Methanosarcina thermophila | |
1.4 | - |
ATP | pH 7.0, 37°C, mutant H180R, produced in E. coli | Methanosarcina thermophila | |
1.5 | - |
ATP | pH 7.0, 37°C, mutant H123A, produced in E. coli | Methanosarcina thermophila | |
1.7 | - |
ATP | pH 7.0, 37°C, mutant H90A, produced in E. coli | Methanosarcina thermophila | |
1.9 | - |
ATP | pH 7.0, 37°C, mutant H208A, produced in E. coli | Methanosarcina thermophila | |
2 | - |
ATP | pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli | Methanosarcina thermophila | |
2.7 | - |
ATP | pH 7.0, 37°C, mutant H184A, produced in E. coli | Methanosarcina thermophila | |
2.8 | - |
ATP | pH 7.0, 37°C, authentic wild-type enzyme | Methanosarcina thermophila | |
3 | - |
ATP | pH 7.0, 37°C, mutant H60A, produced in E. coli | Methanosarcina thermophila | |
3.7 | - |
ATP | pH 7.0, 37°C, mutant H180Q, produced in E. coli | Methanosarcina thermophila | |
4.6 | - |
ATP | pH 7.0, 37°C, mutant H180N, produced in E. coli | Methanosarcina thermophila | |
4.7 | - |
ATP | pH 7.0, 37°C, mutant H180D, produced in E. coli | Methanosarcina thermophila | |
5.8 | - |
ATP | pH 7.0, 37°C, mutant H94A, produced in E. coli | Methanosarcina thermophila | |
6.2 | - |
ATP | pH 7.0, 37°C, mutant H180E, produced in E. coli | Methanosarcina thermophila | |
6.3 | - |
ATP | pH 7.0, 37°C, mutant H180K, produced in E. coli | Methanosarcina thermophila | |
10 | - |
acetate | pH 7.0, 37°C, mutant H180R, produced in E. coli | Methanosarcina thermophila | |
13 | - |
acetate | pH 7.0, 37°C, mutant H180D, produced in E. coli | Methanosarcina thermophila | |
14 | - |
acetate | pH 7.0, 37°C, mutant H180Q, produced in E. coli | Methanosarcina thermophila | |
15 | - |
acetate | pH 7.0, 37°C, mutant H184A, produced in E. coli | Methanosarcina thermophila | |
16 | - |
acetate | pH 7.0, 37°C, mutant H123A, produced in E. coli | Methanosarcina thermophila | |
17 | - |
acetate | pH 7.0, 37°C, mutant H180E, H180N, produced in E. coli | Methanosarcina thermophila | |
18 | - |
acetate | pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli | Methanosarcina thermophila | |
19 | - |
acetate | pH 7.0, 37°C, mutant H180K, produced in E. coli | Methanosarcina thermophila | |
20 | - |
acetate | pH 7.0, 37°C, mutant H60A, produced in E. coli | Methanosarcina thermophila | |
22 | - |
acetate | pH 7.0, 37°C, authentic wild-type enzyme | Methanosarcina thermophila | |
33 | - |
acetate | pH 7.0, 37°C, mutant H208A, produced in E. coli | Methanosarcina thermophila | |
56 | - |
ATP | pH 7.0, 37°C, mutant H180A, produced in E. coli | Methanosarcina thermophila | |
66 | - |
acetate | pH 7.0, 37°C, mutant H94A, produced in E. coli | Methanosarcina thermophila | |
330 | - |
acetate | pH 7.0, 37°C, mutant H90A, produced in E. coli | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |