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Literature summary for 2.7.2.1 extracted from

  • Aceti, D.J.; Ferry, J.G.
    Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila (1988), J. Biol. Chem., 249, 15444-15448.
No PubMed abstract available

General Stability

General Stability Organism
all 4 substrates of the forward and reverse reaction protect the enzyme from inactivation by diethyldicarbonate Methanosarcina thermophila
unaffected by repeated freezing and thawing Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
ATP pH 7.3, 37°C Methanosarcina thermophila
22
-
acetate pH 7.3, 37°C Methanosarcina thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Methanosarcina thermophila 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Methanosarcina thermophila
Co2+
-
Methanosarcina thermophila
Mn2+
-
Methanosarcina thermophila
additional information no activity with Cu2+, Ni2+ or Zn2+ Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, alpha2 homodimer Methanosarcina thermophila
94000
-
gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Methanosarcina thermophila function in the initial activation of acetate for conversion to methane and CO2 ADP + acetyl phosphate
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Oxidation Stability

Oxidation Stability Organism
stable to O2 Methanosarcina thermophila

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
656
-
-
Methanosarcina thermophila

Storage Stability

Storage Stability Organism
-196°C, stable for at least 4 months in liquid nitrogen Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
Methanosarcina thermophila ADP + acetyl phosphate
-
r
ATP + acetate function in the initial activation of acetate for conversion to methane and CO2 Methanosarcina thermophila ADP + acetyl phosphate
-
r
ATP + propionate
-
Methanosarcina thermophila ADP + propionyl phosphate
-
r
CTP + acetate
-
Methanosarcina thermophila CDP + acetyl phosphate
-
r
GTP + acetate
-
Methanosarcina thermophila GDP + acetyl phosphate
-
r
ITP + acetate
-
Methanosarcina thermophila IDP + acetyl phosphate
-
r
additional information formate is no substrate Methanosarcina thermophila ?
-
?
TTP + acetate
-
Methanosarcina thermophila TDP + acetyl phosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 53000, alpha2 homodimer Methanosarcina thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
Methanosarcina thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
stable to heating for 15 min, but rapidly inactivated at higher temperatures Methanosarcina thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.4
-
Methanosarcina thermophila

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 about 35% of maximum activity at pH 5.5 and 9.0 Methanosarcina thermophila

pI Value

Organism Comment pI Value Maximum pI Value
Methanosarcina thermophila
-
4.8 4.7