General Stability | Organism |
---|---|
all 4 substrates of the forward and reverse reaction protect the enzyme from inactivation by diethyldicarbonate | Methanosarcina thermophila |
unaffected by repeated freezing and thawing | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
ATP | pH 7.3, 37°C | Methanosarcina thermophila | |
22 | - |
acetate | pH 7.3, 37°C | Methanosarcina thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Methanosarcina thermophila | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Methanosarcina thermophila | |
Co2+ | - |
Methanosarcina thermophila | |
Mn2+ | - |
Methanosarcina thermophila | |
additional information | no activity with Cu2+, Ni2+ or Zn2+ | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, alpha2 homodimer | Methanosarcina thermophila |
94000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Methanosarcina thermophila | function in the initial activation of acetate for conversion to methane and CO2 | ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Oxidation Stability | Organism |
---|---|
stable to O2 | Methanosarcina thermophila |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
656 | - |
- |
Methanosarcina thermophila |
Storage Stability | Organism |
---|---|
-196°C, stable for at least 4 months in liquid nitrogen | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
ATP + acetate | function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
ATP + propionate | - |
Methanosarcina thermophila | ADP + propionyl phosphate | - |
r | |
CTP + acetate | - |
Methanosarcina thermophila | CDP + acetyl phosphate | - |
r | |
GTP + acetate | - |
Methanosarcina thermophila | GDP + acetyl phosphate | - |
r | |
ITP + acetate | - |
Methanosarcina thermophila | IDP + acetyl phosphate | - |
r | |
additional information | formate is no substrate | Methanosarcina thermophila | ? | - |
? | |
TTP + acetate | - |
Methanosarcina thermophila | TDP + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, alpha2 homodimer | Methanosarcina thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Methanosarcina thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable to heating for 15 min, but rapidly inactivated at higher temperatures | Methanosarcina thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | - |
Methanosarcina thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | about 35% of maximum activity at pH 5.5 and 9.0 | Methanosarcina thermophila |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanosarcina thermophila | - |
4.8 | 4.7 |