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Literature summary for 2.7.13.3 extracted from

  • Ali-Ahmad, A.; Fadel, F.; Sebban-Kreuzer, C.; Ba, M.; Pelissier, G.D.; Bornet, O.; Guerlesquin, F.; Bourne, Y.; Bordi, C.; Vincent, F.
    Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa (2017), Sci. Rep., 7, 11262 .
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
NMR solution structure, the GacS periplasmic domain displays a distinct PDC/PAS alpha/beta fold characterized by a three-stranded beta-sheet flanked by alpha-helices and an atypical major loop Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
H124A no change in overall periplasmic domain fold, mutant shows an altered biofilm morphology compared to the wild-type strain Pseudomonas aeruginosa
H133A no change in overall periplasmic domain fold, mutant abolishes biofilm formation Pseudomonas aeruginosa
H97A mutant abolishes biofilm formation Pseudomonas aeruginosa
R94A mutant strain shows biofilm morphology similar to wild-type Pseudomonas aeruginosa
R94A/H97A no change in overall periplasmic domain fold Pseudomonas aeruginosa
W150A variant is misfolded Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas aeruginosa 16020
-

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa G3XD98
-
-
Pseudomonas aeruginosa DSM 22644 G3XD98
-
-

Synonyms

Synonyms Comment Organism
GacS
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function a GacS mutant devoid of the periplasmic detector domain is severely defective in biofilm formation accompanied by concomitant changes in the expression of small RNAs RsmY/Z that control activation of GacA-regulated genes. Point mutations in a putative ligand binding pocket lined by positively-charged residues originating primarily from the major loop impair biofilm formation Pseudomonas aeruginosa