Literature summary for 2.7.13.3 extracted from

Hofmann, A.; Mueller, S.; Drechsler, T.; Berleth, M.; Caesar, K.; Rohr, L.; Harter, K.; Groth, G.
High-level expression, purification and initial characterization of recombinant Arabidopsis histidine kinase AHK1 (2020), Plants (Basel), 9, 304 .

Search for more literature for 2.7.13.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Arabidopsis thaliana	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
111000	-	gel filtration	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SXL4	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no phosphoprotein	no automodification of AHK1 with ATP is observed	Arabidopsis thaliana

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, 1% (w/v) FosCholine-16 is most efficient in solubilizing the recombinant histidine kinase from the bacterial membranes	Arabidopsis thaliana

Subunits

Subunits	Comment	Organism
monomer	1 * 110000, SDS-PAGE	Arabidopsis thaliana
More	high affinity binding is observed between AHK1 monomers resulting in the formation of the dimer present at physiological conditions. A high affinity interaction is also observed between AHK1 and AHP2 transfer protein	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AHK1	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)