Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.13.3 extracted from

  • Chourashi, R.; Mondal, M.; Sinha, R.; Debnath, A.; Das, S.; Koley, H.; Chatterjee, N.S.
    Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis (2016), Int. J. Med. Microbiol., 306, 657-665.
    View publication on PubMed
Search for more literature for 2.7.13.3

Activating Compound

Activating Compound Comment Organism Structure
mucin the enzyme ChiS is activated 7.4fold in presence of intestinal mucin in vivo and in media supplemented with 2% w/v porcine mucin, the isogenic chiS mutant (ChiS?) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. Activation of ChiS in the host intestine Vibrio cholerae O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000
-
-
 Vibrio cholerae O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae O1 Q9KUA1
-
-
Vibrio cholerae O1 ATCC 39315 Q9KUA1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 wild-type strain in absence of mucin, 37°C, pH not specified in the publication Vibrio cholerae O1
0.1805
-
 wild-type strain in presence of mucin, 37°C, pH not specified in the publication Vibrio cholerae O1

Synonyms

Synonyms Comment Organism
ChiS
-
 Vibrio cholerae O1
sensor histidine kinase
-
 Vibrio cholerae O1
VC0622
-
 Vibrio cholerae O1
VC_0622
-
 Vibrio cholerae O1

General Information

General Information Comment Organism
malfunction the isogenic chiS mutant (ChiS-) shows less growth compared to the wild-type strain ChiS+ in the presence of mucin supplemented media. The mutant ChiS- strain also shows highly retarded motility as well as mucin layer penetration in vitro Vibrio cholerae O1
physiological function role of a sensor histidine kinase ChiS as an regulator in pathogenesis. The enzyme regulates the chitin utilization pathway and the associated two required factors, chitin binding protein and chitinases, like ChiA2. Enzyme ChiS is important for adherence and survival in HT-29 cells. ChiS is an important factor for utilizing mucin as a sole nutrient source. Cell adhesion, motility, and mucin penetration depends on ChiS. ChiS affects suckling mice colonization in mice and contributes in fluid accumulation as well as colonization in rabbit intestine Vibrio cholerae O1