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Literature summary for 2.7.13.3 extracted from

  • Prost, L.R.; Daley, M.E.; Bader, M.W.; Klevit, R.E.; Miller, S.I.
    The PhoQ histidine kinases of Salmonella and Pseudomonas spp. are structurally and functionally different: evidence that pH and antimicrobial peptide sensing contribute to mammalian pathogenesis (2008), Mol. Microbiol., 69, 503-519.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.13.3

Protein Variants

Protein Variants Comment Organism
A143G the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
A143G the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
D110A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
D110A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
D131A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
D131A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
D131A the purified mutant enzyme shows no fluorescence change well into the mM range of Mg2+, suggesting that this mutation renders the protein incapable of binding Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
D131A the purified mutant enzyme shows no fluorescence change well into the mM range of Mg2+, suggesting that this mutation renders the protein incapable of binding Mg2+ Pseudomonas aeruginosa
E115A the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
E115A the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
E133A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
E133A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
E77A the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
E77A the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
G139A the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations Pseudomonas aeruginosa
G139A the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations Salmonella enterica subsp. enterica serovar Typhimurium
L137A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
L137A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
L158A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
L158A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa
S145A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Salmonella enterica subsp. enterica serovar Typhimurium
S145A the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Pseudomonas aeruginosa
Ca2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ PhoQ is strongly repressed in divalent cation-replete medium at 10 mM Mg2+ Pseudomonas aeruginosa
Mg2+ PhoQ is strongly repressed in divalent cation-replete medium at 10 mM Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Salmonella enterica subsp. enterica serovar Typhimurium 16020
-
membrane
-
 Pseudomonas aeruginosa 16020
-

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + PhoN protein L-histidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + PhoN protein N-phospho-L-histidine
-
 ?
ATP + PhoN protein L-histidine
-
 Pseudomonas aeruginosa ADP + PhoN protein N-phospho-L-histidine
-
 ?

Synonyms

Synonyms Comment Organism
PhoQ
-
 Salmonella enterica subsp. enterica serovar Typhimurium
PhoQ
-
 Pseudomonas aeruginosa
sensor histidine kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
sensor histidine kinase
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
ATP
-
 Pseudomonas aeruginosa

Expression

Organism Comment Expression
Salmonella enterica subsp. enterica serovar Typhimurium PhoQ is activated by acidic pH (pH 5.5) up
Pseudomonas aeruginosa PhoQ is activated by acidic pH (pH 5.5) up