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Literature summary for 2.7.13.3 extracted from

  • Filippou, P.S.; Kasemian, L.D.; Panagiotidis, C.A.; Kyriakidis, D.A.
    Functional characterization of the histidine kinase of the E. coli two-component signal transduction system AtoS-AtoC (2008), Biochim. Biophys. Acta, 1780, 1023-1031.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
H398L the mutant retains itscapability to bind ATP and is competent to catalyze the transphosphorylation of an AtoS G-box (G565A) mutant protein which otherwise fails to autophosphorylate due to its inability to bind ATP Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
HCl cytoplasmic phosphorylated AtoS is sensitive to treatment with 1 N HCl but stable in the presence of 1 N NaOH Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Escherichia coli 5737
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation, histidine-398 in the conserved H-box is the phospho-accepting site Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
IMAC and Ni2+-NTA agarose column chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
protein refolding is achieved by dialysis in storage buffer (50 mM Tris-HCl pH 7.6, 0.5 mM dithiothreitol, 50% (v/v) glycerol) containing 0.01% (v/v) Triton X-100 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein L-histidine
-
Escherichia coli ADP + protein N-phospho-L-histidine
-
?

Subunits

Subunits Comment Organism
dimer SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
AtoS
-
Escherichia coli
AtoS-AtoC two-component signal transduction system
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli