Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | light-activated | Brucella abortus | |
additional information | light-activated | Pseudomonas syringae | |
additional information | light-activated | Erythrobacter litoralis | |
additional information | light-activated, 2fold increase in transcription of the LOV-HK gene at a pH similar to that within infected macrophages | Brucella melitensis |
Application | Comment | Organism |
---|---|---|
additional information | a LOV domain at the N-terminus, followed by a PAS domain in the intervening sequence, and a histidine kinase at the C-terminus | Brucella abortus |
additional information | a LOV domain at the N-terminus, followed by a PAS domain in the intervening sequence, and a histidine kinase at the C-terminus | Brucella melitensis |
additional information | LOV domain at the N terminus, followed by a histidine kinase and a putative C-terminal receiver domain | Pseudomonas syringae |
additional information | LOV domain followed by a linking sequence and a C-terminal histidine kinase domain | Erythrobacter litoralis |
Cloned (Comment) | Organism |
---|---|
EL368-LOV-HK and EL346-LOV-HK expressed in Escherichia coli | Erythrobacter litoralis |
LOV-HK expressed in Escherichia coli | Pseudomonas syringae |
LOV-HK expressed in Escherichia coli | Brucella melitensis |
Protein Variants | Comment | Organism |
---|---|---|
C69A | LOV-HK mutant, binds flavin mononucleotide but cannot form the cysteinyl adduct, does not activate the kinase in response to illumination | Brucella melitensis |
C69A | mutant in LOV-HK knockout background does not return the replication rate to the wild-type level | Brucella abortus |
additional information | insertion-knockout mutant strain by introduction of a kanamycin-resistant cassette into the gene, replication is less than the wild-type strain as early as 4 hours after infection with J774A.1 murine macrophages | Brucella abortus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in the dark, roughly an order of magnitude fewer bacteria survive, a survival rate no better than that of the LOV-HK knockout mutant | Brucella abortus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella abortus | - |
- |
- |
Brucella melitensis | - |
- |
- |
Erythrobacter litoralis | - |
- |
- |
Pseudomonas syringae | - |
- |
- |
Pseudomonas syringae DC3000 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
full-length protein affinity-purified | Pseudomonas syringae |
full-length protein affinity-purified | Brucella melitensis |
full-length protein affinity-purified | Erythrobacter litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation | Brucella abortus | ? | - |
? | |
additional information | upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation | Pseudomonas syringae | ? | - |
? | |
additional information | upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation | Brucella melitensis | ? | - |
? | |
additional information | upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation | Erythrobacter litoralis | ? | - |
? | |
additional information | upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation | Pseudomonas syringae DC3000 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
histidine kinase | - |
Brucella abortus |
histidine kinase | - |
Pseudomonas syringae |
histidine kinase | - |
Brucella melitensis |
histidine kinase | - |
Erythrobacter litoralis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Pseudomonas syringae | |
flavin | - |
Erythrobacter litoralis | |
flavin | flavin-containing histidine kinase functions as a photoreceptor, the flavin chromophore, upon illumination, forms a cysteinyl-flavin adduct, which is the signaling state that activates the histidine kinase | Brucella melitensis | |
flavin | flavin-containing histidine kinase functions as a photoreceptor, the flavin chromophore, upon illumination, forms a cysteinyl-flavin adduct, which is the signaling state that activates the histidine kinase, regulates Brucella abortus virulence | Brucella abortus |