Application | Comment | Organism |
---|---|---|
additional information | responsible role of the response regulator TrrA for the feedback regulation of sensing and/or kinase activities of ThkA | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) CodonPlus RIL-X | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
ThkA complexed with the response regulator TrrA, by the batch method, X-ray crystallography at a resolution of 4.2 A, 2fold symmetry, two monomeric TrrAs bind to the ThkA dimer | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
additional information | delta408ThkA, includes the PAS-sensor, the DHp and CA domains, and delta 517ThkA without the PAS-sensor domain, both are fully active in the autophosphorylation reaction | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
delta517ThkA, experimental estimates | Thermotoga maritima |
76000 | - |
delta408ThkA, experimental estimates | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X180 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | autophosphorylation activity | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallography | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
histidine kinase | - |
Thermotoga maritima |
ThkA | - |
Thermotoga maritima |