Cloned (Comment) | Organism |
---|---|
mutant enzymes cloned and expressed in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D286C | t1/2 at room temperature is 16 min, compared to 12 min for wild-type enzyme. 71% of the wild-type phosphatase activity | Escherichia coli |
E257C | t1/2 at room temperature is 30 min, compared to 12 min for wild-type enzyme. 31% of the wild-type phosphatase activity | Escherichia coli |
E261C | t1/2 at room temperature is 15 min, compared to 12 min for wild-type enzyme. 77% of the wild-type phosphatase activity | Escherichia coli |
E268C | t1/2 at room temperature is 23 min, compared to 12 min for wild-type enzyme. 45% of the wild-type phosphatase activity | Escherichia coli |
E275C | t1/2 at room temperature is 28 min, compared to 12 min for wild-type enzyme. 34% of the wild-type phosphatase activity | Escherichia coli |
E276C | t1/2 at room temperature is 68 min, compared to 12 min for wild-type enzyme. 5% of the wild-type phosphatase activity | Escherichia coli |
E282C | t1/2 at room temperature is 15 min, compared to 12 min for wild-type enzyme. 77% of the wild-type phosphatase activity | Escherichia coli |
G240C | t1/2 at room temperature is 10 min, compared to 12 min for wild-type enzyme. 123% of the wild-type phosphatase activity | Escherichia coli |
G264C | t1/2 at room temperature is 12 min, compared to 12 min for wild-type enzyme. As active as wild-type enzyme | Escherichia coli |
H243K | inactive mutant protein | Escherichia coli |
H243N | inactive mutant protein | Escherichia coli |
H243S | inactive mutant protein | Escherichia coli |
H243V | inactive mutant protein | Escherichia coli |
H243Y | inactive mutant protein | Escherichia coli |
K272C | t1/2 at room temperature is 55 min, compared to 12 min for wild-type enzyme. 10% of the wild-type phosphatase activity | Escherichia coli |
L236C | t1/2 at room temperature is 25 min, compared to 12 min for wild-type enzyme. 40% of the wild-type phosphatase activity | Escherichia coli |
L254C | inactive mutant enzyme, t1/2 at room temperature is 90 min, compared to 12 min for wild-type enzyme | Escherichia coli |
N271C | t1/2 at room temperature is 36 min, compared to 12 min for wild-type enzyme. 23% of the wild-type phosphatase activity | Escherichia coli |
N278C | t1/2 at room temperature is 45 min, compared to 12 min for wild-type enzyme. 15% of the wild-type phosphatase activity | Escherichia coli |
Q262C | t1/2 at room temperature is 11 min, compared to 12 min for wild-type enzyme. 111% of the wild-type phosphatase activity | Escherichia coli |
Q283C | t1/2 at room temperature is 13 min, compared to 12 min for wild-type enzyme. 91% of the wild-type phosphatase activity | Escherichia coli |
R246C | t1/2 at room temperature is 62 min, compared to 12 min for wild-type enzyme. 7% of the wild-type phosphatase activity | Escherichia coli |
S242C | t1/2 at room temperature is 39 min, compared to 12 min for wild-type enzyme. 20% of the wild-type phosphatase activity | Escherichia coli |
S260C | t1/2 at room temperature is 13 min, compared to 12 min for wild-type enzyme. 91% of the wild-type phosphatase activity | Escherichia coli |
S269C | t1/2 at room temperature is 20 min, compared to 12 min for wild-type enzyme. 54% of the wild-type phosphatase activity | Escherichia coli |
T235C | t1/2 at room temperature is 14 min, compared to 12 min for wild-type enzyme. 84% of the wild-type phosphatase activity | Escherichia coli |
T247R | inactive mutant protein | Escherichia coli |
T250C | t1/2 at room temperature is 23 min, compared to 12 min for wild-type enzyme. 45% of the wild-type phosphatase activity | Escherichia coli |
T256C | t1/2 at room temperature is 27 min, compared to 12 min for wild-type enzyme. 36% of the wild-type phosphatase activity | Escherichia coli |
Y265C | t1/2 at room temperature is 20 min, compared to 12 min for wild-type enzyme. 54% of the wild-type phosphatase activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
histidine kinase EnvZ | - |
Escherichia coli |