Cloned (Comment) | Organism |
---|---|
gene At3g21220, semi-quantitative reverse transcription-PCR expression analysis, cloning and expression of the GST-fusion enzyme MKK5 in Escherichia coli strain BL21 codon plus. Recombinant overexpression of DHA-tagged MKK5 under control of the CaMV 35S promoter in either Arabidopsis thaliana Col-0 wild-type plants or mkk5 plants | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
protoplast | - |
Arabidopsis thaliana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana | |
NaCl | mitogen-activated protein kinase kinase 5, MKK5, is activated in response to salt stress | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8RXG3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myelin basic protein | - |
Arabidopsis thaliana | ADP + phoshorylated myelin basic protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At3g21220 | - |
Arabidopsis thaliana |
mitogen-activated protein kinase kinase 5 | - |
Arabidopsis thaliana |
MKK5 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | construction of a kinase-dead enzyme mutant | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | expression of iron superoxide dismutases FSD2 and FSD3 is significantly increased in Arabidopsis in response to NaCl treatment but blocked in transgenic MKK5-RNAi plant, mkk5. Overexpression of MKK5 in wild-type plants enhances their tolerance to salt treatments, while the mkk5 mutant exhibits hypersensitivity to salt stress in germination on salt-containing media. The kinase activity of MPK6 is totally turned off in mkk5, whereas the activity of MPK3 is only partially blocked. MKK5-overexpressing plants exhibit salt tolerance phenotypes | Arabidopsis thaliana |
metabolism | te enzyme MKK5 is part of the MAP kinase cascade, including MEKK1, MKK5, and MPK6, that mediates the salt-induced expression of iron superoxide dismutases. MKK5 interactes with the MEKK1 protein that is also involved in the salt-induced FSD signalling pathway. Salt-induced iron superoxide dismutases FSD2 and FSD3 expressions are influenced by MEKK1 via MKK5-MPK6-coupled signalling. MPK6 is also involved in the MKK5-mediated iron superoxide dismutase signalling pathway in salt stress. The MKK5-MPK3/MPK6 pathway is similar to the flagellin-induced MAPK signalling pathway, MKK4/MKK5-MPK3/MPK6 | Arabidopsis thaliana |
physiological function | the enzyme plays a role in MAPK signalling cascades involved in salt stress responses | Arabidopsis thaliana |