Cloned (Comment) | Organism |
---|---|
gene dyrk1A, Dyrk1A and HA-tagged SRp55 are cotransfected into HEK-293FT cells for 48 h, and SRp55 is immunoprecipitated with anti-HA antibodies. Dyrk1A is coimmunoprecipitated by SRp55. SRp55 interacts with Dyrk1A through its RRM domain. DYRK1A and SRp55 coexpression and colocalization in HeLA cell nuclei | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K188R | a dominant negative, inactive Dyrk1A mutant | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Rattus norvegicus | - |
ADP + a phosphoprotein | - |
? | |
ATP + serine/arginine-rich protein 55 | Rattus norvegicus | i.e. splicing factor SRp55, DYRK1a mainly phosphorylates the proline-rich domain of SRp55 | ADP + phospho-serine/arginine-rich protein 55 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q63470 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Rattus norvegicus | ADP + a phosphoprotein | - |
? | |
ATP + serine/arginine-rich protein 55 | i.e. splicing factor SRp55, DYRK1a mainly phosphorylates the proline-rich domain of SRp55 | Rattus norvegicus | ADP + phospho-serine/arginine-rich protein 55 | - |
? | |
ATP + serine/arginine-rich protein 55 | i.e. splicing factor SRp55, DYRK1a mainly phosphorylates the proline-rich domain of SRp55. Dyrk1A phosphorylation sites are Ser280, Ser303, and Ser316 | Rattus norvegicus | ADP + phospho-serine/arginine-rich protein 55 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dual specificity tyrosine-phosphorylated and regulated kinase 1A | - |
Rattus norvegicus |
DYRK1A | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of Dyrk1A does not affect Tau exon 10 inclusion, whereas the expression of dominant negative Dyrk1A, Dyrk1AK188R, which results in the loss of its kinase activity, significantly promotes Tau exon10 inclusion | Rattus norvegicus |
physiological function | enzyme Dyrk1A regulates the pre-mRNA alternative splicing of Tau through splicing factors. Dyrk1A interacts with SRp55 through its RRM domain, phosphorylates its proline-rich domain and inhibits its ability to promote Tau exon 10 inclusion. Upregulation of Dyrk1A disrupts the alternative splicing of Tau exon 10, which encodes the second microtubule-binding repeat. Tau 10 alternative splicing generates Tau isoforms with three- or four-microtubule-binding repeats, named 3R-tau and 4R-tau Dysregulation of the alternative splicing of Tau exon 10 causes several types of neurodegenerative diseases, e.g. neurofibrillary degeneration | Rattus norvegicus |