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Literature summary for 2.7.11.B1 extracted from

  • Wang, B.; Yang, S.; Zhang, L.; He, Z.G.
    Archaeal eukaryote-like serine/threonine protein kinase interacts with and phosphorylates a forkhead-associated-domain-containing protein (2010), J. Bacteriol., 192, 1956-1964.
    View publication on PubMed
Search for more literature for 2.7.11.B1

Cloned(Commentary)

Cloned (Comment) Organism
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 Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
D287A mutant enzyme exhibits very weak activity Sulfurisphaera tokodaii
D314A mutant enzyme exhibits very weak activity Sulfurisphaera tokodaii
K166A mutant enzyme exhibits very weak activity Sulfurisphaera tokodaii
T326A mutant enzyme exhibits a higher level of autophosphorylation activity than the wild-type protein Sulfurisphaera tokodaii
T329A mutant enzyme exhibits very weak activity Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 8% of the activation compared to Mn2+ Sulfurisphaera tokodaii
Mn2+ the enzyme prefers Mn2+ as a cofactor for autophosphorylation and for substrate phosphorylation. Very low levels of activity were observed when several other metal ions, such as Ni2+, Zn2+, Mg2+, and Ca2+ are added Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [forkhead-associated-domain-containing protein ST0829] Sulfurisphaera tokodaii forkhead-associated-domain-containing protein ST0829 is a potential transcriptional regulator. The phosphorylation signal might participate in transcriptional regulation, and may be vital for the extreme acidothermophilic archaeon to exhibit appropriate adaptive responses to environmental cues ADP + [forkhead-associated-domain-containing protein ST0829]phosphate
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 ?
ATP + [forkhead-associated-domain-containing protein ST0829] Sulfurisphaera tokodaii 7 forkhead-associated-domain-containing protein ST0829 is a potential transcriptional regulator. The phosphorylation signal might participate in transcriptional regulation, and may be vital for the extreme acidothermophilic archaeon to exhibit appropriate adaptive responses to environmental cues ADP + [forkhead-associated-domain-containing protein ST0829]phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q970N3
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Sulfurisphaera tokodaii 7 Q970N3
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Purification (Commentary)

Purification (Comment) Organism
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 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [forkhead-associated-domain-containing protein ST0829] forkhead-associated-domain-containing protein ST0829 is a potential transcriptional regulator. The phosphorylation signal might participate in transcriptional regulation, and may be vital for the extreme acidothermophilic archaeon to exhibit appropriate adaptive responses to environmental cues Sulfurisphaera tokodaii ADP + [forkhead-associated-domain-containing protein ST0829]phosphate
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 ?
ATP + [forkhead-associated-domain-containing protein ST0829] Thr329 is part of a major activation site in the kinase, while Thr326 is a negative regulation site. Several mutants with amino acid substitutions in the conserved forkhead-associated-domain sites of ST0829 do not physically interact with the kinase Sulfurisphaera tokodaii ADP + [forkhead-associated-domain-containing protein ST0829]phosphate
-
 ?
ATP + [forkhead-associated-domain-containing protein ST0829] forkhead-associated-domain-containing protein ST0829 is a potential transcriptional regulator. The phosphorylation signal might participate in transcriptional regulation, and may be vital for the extreme acidothermophilic archaeon to exhibit appropriate adaptive responses to environmental cues Sulfurisphaera tokodaii 7 ADP + [forkhead-associated-domain-containing protein ST0829]phosphate
-
 ?
ATP + [forkhead-associated-domain-containing protein ST0829] Thr329 is part of a major activation site in the kinase, while Thr326 is a negative regulation site. Several mutants with amino acid substitutions in the conserved forkhead-associated-domain sites of ST0829 do not physically interact with the kinase Sulfurisphaera tokodaii 7 ADP + [forkhead-associated-domain-containing protein ST0829]phosphate
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 ?

Synonyms

Synonyms Comment Organism
STK_15650 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
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 Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 80 37°C: about 55% of maximal activity, 80°C: about 80% of maximal activity Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5
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 Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 pH 4.5: about 30% of maximal activity, pG 8.5: about 20% of maximal activity Sulfurisphaera tokodaii

General Information

General Information Comment Organism
physiological function forkhead-associated-domain-containing protein ST0829 is a potential transcriptional regulator. The phosphorylation signal might participate in transcriptional regulation, and may be vital for the extreme acidothermophilic archaeon to exhibit appropriate adaptive responses to environmental cues Sulfurisphaera tokodaii