Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged Goodpasture antigen-binding protein in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I439A/W588A | site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P | Homo sapiens |
additional information | immobilization of Goodpasture antigen-binding protein by human serum amyloid P component binding from solution and in plasma, overview. Serum amyloid P associates in the blood with a 37-kDa GPBP fragment containing the N-terminal region and the middle domain | Homo sapiens |
N460A/Y461A | site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P | Homo sapiens |
P500G/A501Q | site-directed mutagenesis, the mutant binding to serum amyloid P is abolished | Homo sapiens |
V498A/W499A | site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P | Homo sapiens |
Y461A/F462A | site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular matrix | - |
Homo sapiens | 31012 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
additional information | binding of collagen IV to Goodpasture antigen-binding protein is independent of the presence of Ca2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [Goodpasture antigen-binding protein] | Homo sapiens | the Goodpasture antigen-binding protein performs phosphorylation of collagen IV | ADP + [Goodpasture antigen-binding phosphoprotein] | - |
? | |
additional information | Homo sapiens | Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y5P4 | isoform 1 | - |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged Goodpasture antigen-binding protein from Escherichia coli strain BL21(DE3) by affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | Goodpasture antigen-binding protein is present in brain amyloid plaques | Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [Goodpasture antigen-binding protein] | - |
Homo sapiens | ADP + [Goodpasture antigen-binding phosphoprotein] | - |
? | |
ATP + [Goodpasture antigen-binding protein] | the Goodpasture antigen-binding protein performs phosphorylation of collagen IV | Homo sapiens | ADP + [Goodpasture antigen-binding phosphoprotein] | - |
? | |
additional information | Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview | Homo sapiens | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | Goodpasture antigen-binding protein, GPBP, is a nonconventional Ser/Thr kinase for basement membrane type IV collagen | Homo sapiens |