BRENDA - Enzyme Database
show all sequences of 2.7.11.9

Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques

Mencarelli, C.; Bode, G.H.; Losen, M.; Kulharia, M.; Molenaar, P.C.; Veerhuis, R.; Steinbusch, H.W.; De Baets, M.H.; Nicolaes, G.A.; Martinez-Martinez, P.; J. Biol. Chem. 287, 14897-14911 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of FLAG-tagged Goodpasture antigen-binding protein in Escherichia coli strain BL21(DE3)
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
I439A/W588A
site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P
Homo sapiens
additional information
immobilization of Goodpasture antigen-binding protein by human serum amyloid P component binding from solution and in plasma, overview. Serum amyloid P associates in the blood with a 37-kDa GPBP fragment containing the N-terminal region and the middle domain
Homo sapiens
N460A/Y461A
site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P
Homo sapiens
P500G/A501Q
site-directed mutagenesis, the mutant binding to serum amyloid P is abolished
Homo sapiens
V498A/W499A
site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P
Homo sapiens
Y461A/F462A
site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular matrix
-
Homo sapiens
31012
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
additional information
binding of collagen IV to Goodpasture antigen-binding protein is independent of the presence of Ca2+
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + [Goodpasture antigen-binding protein]
Homo sapiens
the Goodpasture antigen-binding protein performs phosphorylation of collagen IV
ADP + [Goodpasture antigen-binding phosphoprotein]
-
-
?
additional information
Homo sapiens
Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9Y5P4
isoform 1
-
Purification (Commentary)
Commentary
Organism
recombinant FLAG-tagged Goodpasture antigen-binding protein from Escherichia coli strain BL21(DE3) by affinity chromatography
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
Goodpasture antigen-binding protein is present in brain amyloid plaques
Homo sapiens
-
plasma
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + [Goodpasture antigen-binding protein]
-
722751
Homo sapiens
ADP + [Goodpasture antigen-binding phosphoprotein]
-
-
-
?
ATP + [Goodpasture antigen-binding protein]
the Goodpasture antigen-binding protein performs phosphorylation of collagen IV
722751
Homo sapiens
ADP + [Goodpasture antigen-binding phosphoprotein]
-
-
-
?
additional information
Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview
722751
Homo sapiens
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of FLAG-tagged Goodpasture antigen-binding protein in Escherichia coli strain BL21(DE3)
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
I439A/W588A
site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P
Homo sapiens
additional information
immobilization of Goodpasture antigen-binding protein by human serum amyloid P component binding from solution and in plasma, overview. Serum amyloid P associates in the blood with a 37-kDa GPBP fragment containing the N-terminal region and the middle domain
Homo sapiens
N460A/Y461A
site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P
Homo sapiens
P500G/A501Q
site-directed mutagenesis, the mutant binding to serum amyloid P is abolished
Homo sapiens
V498A/W499A
site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P
Homo sapiens
Y461A/F462A
site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular matrix
-
Homo sapiens
31012
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
additional information
binding of collagen IV to Goodpasture antigen-binding protein is independent of the presence of Ca2+
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + [Goodpasture antigen-binding protein]
Homo sapiens
the Goodpasture antigen-binding protein performs phosphorylation of collagen IV
ADP + [Goodpasture antigen-binding phosphoprotein]
-
-
?
additional information
Homo sapiens
Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant FLAG-tagged Goodpasture antigen-binding protein from Escherichia coli strain BL21(DE3) by affinity chromatography
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
Goodpasture antigen-binding protein is present in brain amyloid plaques
Homo sapiens
-
plasma
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + [Goodpasture antigen-binding protein]
-
722751
Homo sapiens
ADP + [Goodpasture antigen-binding phosphoprotein]
-
-
-
?
ATP + [Goodpasture antigen-binding protein]
the Goodpasture antigen-binding protein performs phosphorylation of collagen IV
722751
Homo sapiens
ADP + [Goodpasture antigen-binding phosphoprotein]
-
-
-
?
additional information
Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview
722751
Homo sapiens
?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
Goodpasture antigen-binding protein, GPBP, is a nonconventional Ser/Thr kinase for basement membrane type IV collagen
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Goodpasture antigen-binding protein, GPBP, is a nonconventional Ser/Thr kinase for basement membrane type IV collagen
Homo sapiens
Other publictions for EC 2.7.11.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737750
Hanada
Co-evolution of sphingomyelin ...
Homo sapiens
Biochim. Biophys. Acta
1841
704-719
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722751
Mencarelli
Goodpasture antigen-binding pr ...
Homo sapiens
J. Biol. Chem.
287
14897-14911
2012
-
-
1
-
6
-
-
-
1
2
-
2
-
1
-
-
1
-
-
2
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
2
-
2
-
-
-
1
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722724
Revert-Ros
Goodpasture antigen-binding pr ...
Mus musculus
J. Biol. Chem.
286
35030-35043
2011
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722667
Miralem
Human biliverdin reductase sup ...
Homo sapiens
J. Biol. Chem.
285
12551-12558
2010
1
-
1
-
-
-
1
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
1
1
1
-
-
704804
Mencarelli
The expression of the Goodpast ...
Rattus norvegicus
J. Chem. Neuroanat.
38
97-105
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693093
Granero-Molto
Goodpasture antigen-binding pr ...
Danio rerio
J. Biol. Chem.
283
20495-20504
2008
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693162
Revert
Goodpasture antigen-binding pr ...
Homo sapiens
J. Biol. Chem.
283
30246-30255
2008
-
-
1
-
-
-
-
-
5
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661708
Granero
A human-specific TNF-responsiv ...
Homo sapiens
FEBS J.
272
5291-5305
2005
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
491667
Kawai
Functional annotation of a ful ...
Mus musculus
Nature
409
685-690
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
491668
Raya
Goodpasture antigen-binding pr ...
Bos taurus, Homo sapiens, Mus musculus
J. Biol. Chem.
275
40392-40399
2000
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
491669
Raya
Characterization of a novel ty ...
Homo sapiens
J. Biol. Chem.
274
12642-12649
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-