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Literature summary for 2.7.11.7 extracted from

  • Yang, Y.; Ye, Q.; Jia, Z.; Cote, G.P.
    Characterization of the catalytic and nucleotide binding properties of the alpha-kinase domain of Dictyostelium myosin-II heavy chain kinase A (2015), J. Biol. Chem., 290, 23935-23946.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Dictyostelium discoideum

Crystallization (Commentary)

Crystallization (Comment) Organism
alpha-kinase domain in complex with AMP and adenosine, hanging drop vapor diffusion method, using 200 mM NaH2PO4, 20% (w/v) polyethylene glycol 8000, and 100 mM Tris-HCl, pH 7.5 Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
D663A the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
D756A inactive Dictyostelium discoideum
D766S inactive Dictyostelium discoideum
E713A the mutant shows about 10% activity compared to the wild type enzyme Dictyostelium discoideum
E713D the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
F720S inactive Dictyostelium discoideum
K645R the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
K722N the mutant shows about 20% activity compared to the wild type enzyme Dictyostelium discoideum
L716S the mutant shows about 1% activity compared to the wild type enzyme Dictyostelium discoideum
Q758A the mutant shows about 3% activity compared to the wild type enzyme Dictyostelium discoideum
Q768A the mutant shows about 70% activity compared to the wild type enzyme Dictyostelium discoideum
R592L the mutant shows about 30% activity compared to the wild type enzyme Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 ATP mutant enzyme R592L, at pH 7.0 and 22°C Dictyostelium discoideum
0.063
-
 ATP mutant enzyme K722N, at pH 7.0 and 22°C Dictyostelium discoideum
0.23
-
 ATP wild type enzyme, at pH 7.0 and 22°C Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin heavy-chain] Dictyostelium discoideum
-
 ADP + [myosin heavy-chain] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum P42527
-
-

Purification (Commentary)

Purification (Comment) Organism
DE53 column chromatography and SP-Sepharose column chromatography Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AAYKTKKKK
-
 Dictyostelium discoideum ?
-
 ?
ATP + myelin basic protein
-
 Dictyostelium discoideum ?
-
 ?
ATP + RKKFGESEKTKTKEFL
-
 Dictyostelium discoideum ?
-
 ?
ATP + [myosin heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin heavy-chain] phosphate
-
 ?

Synonyms

Synonyms Comment Organism
A-CAT alpha-kinase domain of myosin II heavy chain kinase A Dictyostelium discoideum
MHCK-A
-
 Dictyostelium discoideum
myosin II heavy chain kinase A
-
 Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 ATP mutant enzyme K722N, at pH 7.0 and 22°C Dictyostelium discoideum
0.03
-
 ATP mutant enzyme R592L, at pH 7.0 and 22°C Dictyostelium discoideum
0.1
-
 ATP wild type enzyme, at pH 7.0 and 22°C Dictyostelium discoideum