Activating Compound | Comment | Organism | Structure |
---|---|---|---|
F-actin | about 40fold activation of myosin heavy chain kinase A, structure and mechanism, stimulation via enzyme autophosphorylation | Dictyostelium discoideum | |
G-actin | slight activation of MHCK A, activation is abolished by latrunculin A, which depolymerizes the actin filament | Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Dictyostelium discoideum, overexpression of isolated coiled-coil domain and of the catalytic domain in Escherichia coli as GST-fused proteins | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the truncation mutant DELTAcoil-MHCK A comprising residues 1-498 is not affected in its autophosphorylation rate by F-actin | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Dictyostelium discoideum | |
KCl | activates | Dictyostelium discoideum | |
Mg2+ | - |
Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin-heavy-chain] | Dictyostelium discoideum | - |
ADP + [myosin-heavy-chain]phosphate | - |
? | |
additional information | Dictyostelium discoideum | biochemical mechanism for the spatial regulation of myosin II filament disassembly acts via MHCK A activation by actin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | P42527 | isozyme MHCK A; isozyme MHCK A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | MHCK A performs autophosphorylation, facilitated by F-actin requiring the coiled coil domain of the enzyme | Dictyostelium discoideum |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A from Dictyostelium discoideum to homogeneity by affinity chromatography, recombinant GST-fusion coiled-coil domain and catalytic domain from Escherichia coli to near homogeneity by affinity chromatography | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin-heavy-chain] | - |
Dictyostelium discoideum | ADP + [myosin-heavy-chain]phosphate | - |
? | |
additional information | biochemical mechanism for the spatial regulation of myosin II filament disassembly acts via MHCK A activation by actin | Dictyostelium discoideum | ? | - |
? | |
additional information | MHCK A performs autophosphorylation | Dictyostelium discoideum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MHCK A | - |
Dictyostelium discoideum |
myosin heavy chain kinase A | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dictyostelium discoideum |