Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.7 extracted from

  • Brzeska, H.; Young, R.; Knaus, U.; Korn, E.D.
    Myosin I heavy chain kinase: cloning of the full-length gene and acidic lipid-dependent activation by Rac and Cdc42 (1999), Proc. Natl. Acad. Sci. USA, 96, 394-399.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by phosphatidylcholine and phosphatidylethanolamine Acanthamoeba castellanii
phosphatidylinositol
-
Acanthamoeba castellanii
phosphatidylserine
-
Acanthamoeba castellanii
Rac linoleic acid supports activation by Rac Acanthamoeba castellanii
Rac activation of autophosphorylation and kinase activity only in presence of phosphatidylserine, activates only the fully phosphorylated enzyme Acanthamoeba castellanii

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Acanthamoeba castellanii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Acanthamoeba castellanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79300
-
x * 79300, determined from amino acid sequence, difference to MW of 97 kDa determined by SDS-PAGE is due to high proline content Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin I heavy chain Acanthamoeba castellanii
-
ADP + myosin I heavy chain phosphate
-
?

Organism

Organism UniProt Comment Textmining
Acanthamoeba castellanii Q93107 purified myosin I heavy chain kinase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GRGRSSVYS synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC Acanthamoeba castellanii ADP + GRGRSS(-phosphate)VYS
-
?
ATP + myosin I heavy chain
-
Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain substrate is heavy chain of myosin IC Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
additional information enzyme contains several SH3-binding domains Acanthamoeba castellanii ?
-
?
additional information enzyme possesses binding domains for the Ras-related GTP-binding proteins Cdc42 and Rac Acanthamoeba castellanii ?
-
?

Subunits

Subunits Comment Organism
? x * 79300, determined from amino acid sequence, difference to MW of 97 kDa determined by SDS-PAGE is due to high proline content Acanthamoeba castellanii

Synonyms

Synonyms Comment Organism
MIHCK
-
Acanthamoeba castellanii
More member of the p21-activated kinase family Acanthamoeba castellanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Acanthamoeba castellanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Acanthamoeba castellanii

Cofactor

Cofactor Comment Organism Structure
ATP
-
Acanthamoeba castellanii