Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by phosphatidylcholine and phosphatidylethanolamine | Acanthamoeba castellanii | |
phosphatidylinositol | - |
Acanthamoeba castellanii | |
phosphatidylserine | - |
Acanthamoeba castellanii | |
Rac | linoleic acid supports activation by Rac | Acanthamoeba castellanii | |
Rac | activation of autophosphorylation and kinase activity only in presence of phosphatidylserine, activates only the fully phosphorylated enzyme | Acanthamoeba castellanii |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Acanthamoeba castellanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acanthamoeba castellanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79300 | - |
x * 79300, determined from amino acid sequence, difference to MW of 97 kDa determined by SDS-PAGE is due to high proline content | Acanthamoeba castellanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | Q93107 | purified myosin I heavy chain kinase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GRGRSSVYS | synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC | Acanthamoeba castellanii | ADP + GRGRSS(-phosphate)VYS | - |
? | |
ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate is heavy chain of myosin IC | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
additional information | enzyme contains several SH3-binding domains | Acanthamoeba castellanii | ? | - |
? | |
additional information | enzyme possesses binding domains for the Ras-related GTP-binding proteins Cdc42 and Rac | Acanthamoeba castellanii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79300, determined from amino acid sequence, difference to MW of 97 kDa determined by SDS-PAGE is due to high proline content | Acanthamoeba castellanii |
Synonyms | Comment | Organism |
---|---|---|
MIHCK | - |
Acanthamoeba castellanii |
More | member of the p21-activated kinase family | Acanthamoeba castellanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acanthamoeba castellanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acanthamoeba castellanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acanthamoeba castellanii |