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Literature summary for 2.7.11.7 extracted from
- Regulation of the p21-activated kinase-related Dictyostelium myosin I heavy chain kinase by autophosphorylation, acidic phospholipids, and Ca2+-calmodulin (1998), J. Biol. Chem., 273, 27911-27917.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acidic phospholipids | functions cooperatively with acidic phospholipids to associate the enzyme with membranes | Dictyostelium discoideum | |
| acidic phospholipids | enhance the activation of the enzyme by autophosphorylation | Dictyostelium discoideum | |
| guanosine 5'-3-O-(thio)triphosphate-Rac1 | functions cooperatively with acidic phospholipids to associate the enzyme with membranes | Dictyostelium discoideum | |
| guanosine 5'-3-O-(thio)triphosphate-Rac1 | i.e. GTPgammaS-Rac1 | Dictyostelium discoideum | |
| guanosine 5'-3-O-(thio)triphosphate-Rac1 | 10fold activation of autophosphorylation and kinase activity | Dictyostelium discoideum | |
| additional information | enzyme is stimulated by its autophosphorylation | Dictyostelium discoideum | |
| additional information | no activation by phosphatidylcholine and sphingosine | Dictyostelium discoideum | |
| phosphatidylinositol | 10fold activation of autophosphorylation and kinase activity | Dictyostelium discoideum |  |
| phosphatidylinositol 4,5-bisphosphate | 10fold activation of autophosphorylation and kinase activity | Dictyostelium discoideum | |
| phosphatidylserine | 10fold activation of autophosphorylation and kinase activity | Dictyostelium discoideum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis | Dictyostelium discoideum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+/calmodulin | binds to the enzyme and inhibits its activation by acidic phospholipids but not by guanosine 5'-3-O-(thio)triphosphate-Rac1 | Dictyostelium discoideum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
GRSARVSTYA | pH 7.0 | Dictyostelium discoideum | |
| 0.017 | - |
GRSARVSTYA | in presence of guanosine 5'-3-O-(thio)triphosphate-Rac1, pH 7.0 | Dictyostelium discoideum | |
| 0.019 | - |
GRSARVSTYA | in presence of phosphatidylserine, pH 7.0 | Dictyostelium discoideum | |
| 0.098 | - |
AKRVSMMR | in presence of guanosine 5'-3-O-(thio)triphosphate-Rac1, pH 7.0 | Dictyostelium discoideum | |
| 0.116 | - |
AKRVSMMR | in presence of phosphatidylserine, pH 7.0 | Dictyostelium discoideum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | association of the enzyme to membranes enhances the activity | Dictyostelium discoideum | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin I heavy chain | Dictyostelium discoideum | - |
ADP + myosin I heavy chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | Q869N2 | myosin I heavy chain kinase | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate | major phosphorylation site: Ser8 | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AKRVSMMR | peptide derived from myosin I heavy chain kinase amino acid residues 4-11, exchange at position 4: Ser to Ala | Dictyostelium discoideum | ADP + AKRVS(-phosphate)MMR | phophorylation site is Ser8 | ? | |
| ATP + GRSARVSTYA | peptide derived from Dictyostelium myosin ID | Dictyostelium discoideum | ADP + GRSARVS(-phosphate)TYA | - |
? | |
| ATP + myosin heavy chain kinase | enzyme incorporates 1 mol of phosphate per mol of enzyme | Dictyostelium discoideum | ADP + myosin heavy chain kinase phosphate | - |
? | |
| ATP + myosin I heavy chain | - |
Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | major site of phosphorylation is Ser8 | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | substrate: myosin ID | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MIHCK | - |
Dictyostelium discoideum |
| More | member of the p21-activated kinase family | Dictyostelium discoideum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
GRSARVSTYA | pH 7.0 | Dictyostelium discoideum | |
| 4.6 | - |
GRSARVSTYA | in presence of guanosine 5'-3-O-(thio)triphosphate-Rac1, pH 7.0 | Dictyostelium discoideum | |
| 4.7 | - |
GRSARVSTYA | in presence of phosphatidylserine, pH 7.0 | Dictyostelium discoideum | |
| 5 | - |
AKRVSMMR | in presence of guanosine 5'-3-O-(thio)triphosphate-Rac1, pH 7.0 | Dictyostelium discoideum | |
| 5.4 | - |
AKRVSMMR | in presence of phosphatidylserine, pH 7.0 | Dictyostelium discoideum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Dictyostelium discoideum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Dictyostelium discoideum | |
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