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Literature summary for 2.7.11.7 extracted from

  • Rico, M.; Egelhoff, T.T.
    Myosin heavy chain kinase B participates in the regulation of myosin assembly into the cytoskeleton (2003), J. Cell. Biochem., 88, 521-532.
    View publication on PubMed
Search for more literature for 2.7.11.7

Cloned(Commentary)

Cloned (Comment) Organism
MHCK B, overexpression as FLAG-tagged protein in AX2 cells or 3xALA cells of Dictyostelium discoideum Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 RKKFGESEKTKTKEFL recombinant MHCK B, pH 7.0, 22°C Dictyostelium discoideum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin II heavy chain Dictyostelium discoideum involved in regulation of myosin II filament assembly ADP + myosin II heavy chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum P90648 MHCK B
-

Purification (Commentary)

Purification (Comment) Organism
MHCK B, recombinant from Dictyostelium discoideum cells as FLAG-tagged protein Dictyostelium discoideum

Reaction

Reaction Comment Organism Reaction ID
ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate enzyme contains the C-terminal WD-domain, responsible for substrate binding, the catalytic domain, and the N-terminal coiled-coil translocation domain Dictyostelium discoideum
a

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Dictyostelium discoideum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myosin II heavy chain isozyme MHCK B Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
ATP + myosin II heavy chain drives filament disassembly in vitro Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
ATP + myosin II heavy chain involved in regulation of myosin II filament assembly Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
ATP + RKKFGESEKTKTKEFL isozyme MHCK B Dictyostelium discoideum ?
-
 ?
ATP + RKKFGESEKTKTKEFL synthetic peptide MH1 Dictyostelium discoideum ?
-
 ?
additional information the Mg2+-ATPase activity of the substrate myosin II is inhibited in vitro by its phosphorylation Dictyostelium discoideum ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum