Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli NM522 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, gel filtration, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Escherichia coli |
Storage Stability | Organism |
---|---|
purified protein stable for at least a week when stored on ice | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate synthase + ADP | His residue phosphorylated (active) | Escherichia coli | [phosphoenolpyruvate synthase]phosphate + AMP | His and Thr residues phosphorylated (inactive) | ? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25000, gel filtration, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
bifunctional ADP-dependent kinase - Pi-dependent pyrophosphorylase | - |
Escherichia coli |
DUF299 | - |
Escherichia coli |
PEP synthetase regulatory protein | - |
Escherichia coli |
PSRP | - |
Escherichia coli |