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Literature summary for 2.7.11.32 extracted from

  • Burnell, J.; Hatch, M.
    Regulation of C4 photosynthesis: Purification and properties of the protein catalyzing ADP-mediated inactivation and Pi-mediated activation of pyruvate,Pi dikinase (1985), Arch. Biochem. Biophys., 237, 490-503.
    View publication on PubMed
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General Stability

General Stability Organism
stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue Zea mays

Inhibitors

Inhibitors Comment Organism Structure
ADP-beta-S competitive to ADP Zea mays
pyruvate,Pi dikinase phosphorylated at a Thr residue competitive to ADP; competitive to substrate pyruvate,Pi dikinase Zea mays
[pyruvate,Pi dikinase]phosphate competitive to substrate pyruvate,Pi dikinase; noncompetitive to ADP Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 pyruvate,Pi dikinase pyruvate,Pi dikinase phosphorylated at a His residue, pH 8.3, temperature not specified in the publication Zea mays
0.052
-
 ADP pH 8.3, temperature not specified in the publication Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Zea mays
90000
-
 pH 7.5, gel filtration Zea mays
180000
-
 pH 8.3, gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 cv. Dekalb XL81
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate,Pi dikinase + ADP substrate pyruvate,Pi dikinase is phosphorylated at a His residue and active; CDP shows 40% activity, GDP 35% activity and UDP 0% activity in comparison with ADP Zea mays [pyruvate,Pi dikinase]phosphate + AMP phosphorylated at a Thr residue, inactive ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
pyruvate,Pi dikinase regulatory protein bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 broad optimum pH 7.8-8.4, 68% activity at pH 7.2 Zea mays