Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-aminoimidazole-4-carboxamide riboside | AICAR, increases phosphorylation of acetyl-CoA carboxylase and AMPK in INS832/13 cells | Rattus norvegicus | |
Diethylamine NONOate | nitric oxide donor, stimulates rapid and transient AMPK phosphorylation in INS832/13 cells and islets | Rattus norvegicus | |
interleukin-1 | induces nitric oxide-dependent activation of AMPK | Rattus norvegicus | |
nitric oxide | AMPK is transiently activated by nitric oxide in insulinoma cells and rat islets following interleukin-1 treatment or by the exogenous addition of nitric oxide | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
adenoviral expression of wild-type, dominant negative or constitutively active AMPK mutants. Transduction of INS832/13 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H150R | constitutively active AMPKgamma1 mutant, stimulates the concentration-dependent increase of substrate phosphorylation | Rattus norvegicus |
K45R | dominant negative AMPKalpha2 mutant, results in a concentration-dependent inhibition in the phosphorylation of the AMPK substrate, inhibits endogenous AMPK activation. Inhibition of AMPK attenuates recovery of aconitase activity and promotes caspase-3 activation during recovery | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
insulinoma cell | - |
Rattus norvegicus | - |
additional information | islet, INS832/13 cells | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA carboxylase | - |
Rattus norvegicus | ADP + [acetyl-CoA carboxylase]phosphate | - |
? | |
additional information | AMPK promotes reactivation of mitochondrial aconitase | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMP-activated protein kinase | - |
Rattus norvegicus |
AMPK | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | active AMPK promotes the functional recovery of beta-cell oxidative metabolism and abrogates the induction of pathways that mediate cell death such as caspase-3 activation following exposure to nitric oxide | Rattus norvegicus |