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Literature summary for 2.7.11.31 extracted from

  • Quinn, J.M.; Tam, S.; Sims, N.A.; Saleh, H.; McGregor, N.E.; Poulton, I.J.; Scott, J.W.; Gillespie, M.T.; Kemp, B.E.; van Denderen, B.J.
    Germline deletion of AMP-activated protein kinase beta subunits reduces bone mass without altering osteoclast differentiation or function (2010), FASEB J., 24, 275-285.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.31

Activating Compound

Activating Compound Comment Organism Structure
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside activates AMPK in BMMs and RAW264.7 cells. While 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside greatly stimulates osteoclast formation, it acts through an AMPK-independent mechanism Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
A134974 at 1 nM ablates the stimulatory action of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside with no effects on osteoclast formation in the absence of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside Mus musculus
additional information osteoclasts and macrophages generated from AMPK beta1-/- mice display no detectable AMPK activity Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 C57BL/6J mice
-
Mus musculus C57/BL6J
-
 C57BL/6J mice
-

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
 Mus musculus
-
additional information present in BMM cells. Osteoclasts and macrophages lack AMPK beta2 subunit expression Mus musculus
-
osteoblast
-
 Mus musculus
-
RAW-264.7 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA carboxylase
-
 Mus musculus ADP + [acetyl-CoA carboxylase]phosphate
-
 ?
ATP + acetyl-CoA carboxylase
-
 Mus musculus C57/BL6J ADP + [acetyl-CoA carboxylase]phosphate
-
 ?

Subunits

Subunits Comment Organism
heterotrimer immunoprecipitation Mus musculus

Synonyms

Synonyms Comment Organism
AMP-activated protein kinase
-
 Mus musculus
AMPK
-
 Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.048
-
-
 Mus musculus A134974

Expression

Organism Comment Expression
Mus musculus loss of AMPK beta-subunit expression in beta1-/- and beta2-/- mice, leading to a low-bone-mass phenotype down

General Information

General Information Comment Organism
malfunction germline deletion of either AMPK beta1 or beta2 subunit isoforms results in reduced trabecular bone density and mass, but without effects on osteoclast or osteoblast numbers, as compared to wild-type littermate controls Mus musculus
physiological function AMPK is required to maintain normal bone density, but not through bone cell differentiation, and does not mediate powerful osteolytic effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside Mus musculus