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Literature summary for 2.7.11.31 extracted from

  • Boyle, J.G.; Logan, P.J.; Ewart, M.; Reihill, J.A.; Ritchie, S.A.; Connell, J.M.; Cleland, S.J.; Salt, I.P.
    Rosiglitazone stimulates nitric oxide synthesis in human aortic endothelial cells via AMP-activated protein kinase (2008), J. Biol. Chem., 283, 11210-11217.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP AMP-binding to AMPK inhibits dephosphorylation at Thr172 Homo sapiens
additional information activation of AMPK requires phosphorylation at Thr172 by an AMPK kinases, e.g. LKB1 and Ca2+/calmodulin-dependent kinase kinase, CaMKK Homo sapiens
rosiglitazone stimulates an increase in the ADP/ATP ratio and AMPK activity essentially involving LKB1 and leading to activation of nitric oxide synthesis in human aortic endothelial cells, the stimulation of AMPK and NO synthesis by rosiglitazone is unaffected by the peroxisome proliferator-activated receptor-gamma inhibitor GW9662 or by STO-609, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzyme in human aortic endothelial cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information a dominant negative AMP-activated protein kinase mutant abrogates rosiglitazone-stimulated Ser1177 phosphorylation and NO production in endothelial cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [endothelial nitic oxide synthase] Homo sapiens activates nitric oxide synthesis, mechanism, overview ADP + [endothelial nitic oxide synthase] phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein activation of AMPK requires phosphorylation at Thr172 by an AMPK kinases, e.g. LKB1 and Ca2+/calmodulin-dependent kinase kinase, CaMKK Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
Homo sapiens
-
endothelial cell human umbilical vein endothelial cell, HUVEC Homo sapiens
-
HeLa cell HeLa cells stably expressing wild type LKB1 or a kinase-inactive mutant LKB1 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [endothelial nitic oxide synthase] activates nitric oxide synthesis, mechanism, overview Homo sapiens ADP + [endothelial nitic oxide synthase] phosphate
-
?
ATP + [endothelial nitic oxide synthase] phosphorylation by AMPK at Ser1177 Homo sapiens ADP + [endothelial nitic oxide synthase] phosphate
-
?

Synonyms

Synonyms Comment Organism
AMP-activated protein kinase
-
Homo sapiens
AMPK
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens