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Literature summary for 2.7.11.31 extracted from

  • Crawford, R.M.; Treharne, K.J.; Best, O.G.; Muimo, R.; Riemen, C.E.; Mehta, A
    A novel physical and functional association between nucleoside diphosphate kinase A and AMP-activated protein kinase alpha1 in liver and lung (2005), Biochem. J., 392, 201-209.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged isozymes alpha1 and alpha2 in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information complex formation between isozyme alpha1 and NDPK-H1 inhibits the AMPK activity, inhibition by NDPK is reduced by addition of ADP or GTP, overview Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
x * 180000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetyl-CoA carboxylase Homo sapiens
-
ADP + phosphorylated acetyl-CoA carboxylase
-
?
ATP + acetyl-CoA carboxylase 1 Rattus norvegicus
-
ADP + phosphorylated acetyl-CoA carboxylase 1
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozymes alpha1 and alpha2
-
Rattus norvegicus
-
isozymes alpha1 and alpha2
-

Purification (Commentary)

Purification (Comment) Organism
isozymes alpha1 and alpha2 from liver homogenates by subcellular fractionation and immunoprecipitation Rattus norvegicus
recombinant His-tagged isozymes alpha1 and alpha2 from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
lung
-
Homo sapiens
-
lung
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA carboxylase
-
Homo sapiens ADP + phosphorylated acetyl-CoA carboxylase
-
?
ATP + acetyl-CoA carboxylase 1
-
Rattus norvegicus ADP + phosphorylated acetyl-CoA carboxylase 1
-
?
ATP + HMRSAMSGLHLVKRR acetyl-CoA carboxylase-derived synthetic peptide substrate Homo sapiens ADP + ?
-
?
ATP + HMRSAMSGLHLVKRR acetyl-CoA carboxylase-derived synthetic peptide substrate Rattus norvegicus ADP + ?
-
?
additional information the enzyme is regulated by the nucleoside diphosphate kinase, complex formation in vivo, e.g. between isozyme alpha1 and NDPK-H1, inhibits the enzyme, overview Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 180000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
AMP-activated protein kinase alpha
-
Rattus norvegicus
AMP-activated protein kinase alpha1
-
Homo sapiens
AMPK
-
Rattus norvegicus
AMPK1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens
30
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Rattus norvegicus