Cloned (Comment) | Organism |
---|---|
expression of His-tagged isozymes alpha1 and alpha2 in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | complex formation between isozyme alpha1 and NDPK-H1 inhibits the AMPK activity, inhibition by NDPK is reduced by addition of ADP or GTP, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
x * 180000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA carboxylase | Homo sapiens | - |
ADP + phosphorylated acetyl-CoA carboxylase | - |
? | |
ATP + acetyl-CoA carboxylase 1 | Rattus norvegicus | - |
ADP + phosphorylated acetyl-CoA carboxylase 1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes alpha1 and alpha2 | - |
Rattus norvegicus | - |
isozymes alpha1 and alpha2 | - |
Purification (Comment) | Organism |
---|---|
isozymes alpha1 and alpha2 from liver homogenates by subcellular fractionation and immunoprecipitation | Rattus norvegicus |
recombinant His-tagged isozymes alpha1 and alpha2 from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Homo sapiens | - |
lung | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA carboxylase | - |
Homo sapiens | ADP + phosphorylated acetyl-CoA carboxylase | - |
? | |
ATP + acetyl-CoA carboxylase 1 | - |
Rattus norvegicus | ADP + phosphorylated acetyl-CoA carboxylase 1 | - |
? | |
ATP + HMRSAMSGLHLVKRR | acetyl-CoA carboxylase-derived synthetic peptide substrate | Homo sapiens | ADP + ? | - |
? | |
ATP + HMRSAMSGLHLVKRR | acetyl-CoA carboxylase-derived synthetic peptide substrate | Rattus norvegicus | ADP + ? | - |
? | |
additional information | the enzyme is regulated by the nucleoside diphosphate kinase, complex formation in vivo, e.g. between isozyme alpha1 and NDPK-H1, inhibits the enzyme, overview | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 180000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
AMP-activated protein kinase alpha | - |
Rattus norvegicus |
AMP-activated protein kinase alpha1 | - |
Homo sapiens |
AMPK | - |
Rattus norvegicus |
AMPK1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus |