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show all sequences of 2.7.11.3

Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase

Hawley, S.A.; Davison, M.; Woods, A.; Davies, S.P.; Beri, R.K.; Carling, D.; Hardie, D.G.; J. Biol. Chem. 271, 27879-27887 (1996)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
5'-AMP
-
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
? * 58000 + ?, catalytic alpha subunit, SDS-PAGE
Rattus norvegicus
196000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
Rattus norvegicus
phosphorylation activates EC 2.7.1.109
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
101
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, can be stored in buffer containing 50% glycerol for up to a month
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644977
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
phosphorylation activates EC 2.7.1.109
644977
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
? * 58000 + ?, catalytic alpha subunit, SDS-PAGE
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
5'-AMP
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
? * 58000 + ?, catalytic alpha subunit, SDS-PAGE
Rattus norvegicus
196000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
Rattus norvegicus
phosphorylation activates EC 2.7.1.109
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
101
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, can be stored in buffer containing 50% glycerol for up to a month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
644977
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
phosphorylation activates EC 2.7.1.109
644977
Rattus norvegicus
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
? * 58000 + ?, catalytic alpha subunit, SDS-PAGE
Rattus norvegicus
Other publictions for EC 2.7.11.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
644998
Hamilton
AMP-activated protein kinase k ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
293
892-898
2002
-
-
1
-
1
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-
-
-
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1
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2
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-
-
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2
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1
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1
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-
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1
-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644977
Hawley
Characterization of the AMP-ac ...
Rattus norvegicus
J. Biol. Chem.
271
27879-27887
1996
1
-
-
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
2
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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2
1
-
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-
1
-
2
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644976
Hawley
5'-AMP activates the AMP-activ ...
Rattus norvegicus
J. Biol. Chem.
270
27186-27191
1995
-
-
-
-
-
-
-
-
-
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2
-
1
-
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1
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3
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2
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-
1
-
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3
-
-
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-
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-
-
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-
-
-
-
-
644972
Weekes
Activation of rat liver AMP-ac ...
Rattus norvegicus
Eur. J. Biochem.
219
751-757
1994
3
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
1
-
-
1
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-
3
-
1
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-
-
-
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-
1
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3
-
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1
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1
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1
1
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1
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1
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1
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3
-
1
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644973
Beg
In vivo modulation of rat live ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Proc. Natl. Acad. Sci. USA
81
7293-7297
1984
1
-
-
-
-
-
-
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1
1
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2
-
162
-
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1
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2
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4
-
1
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1
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1
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1
1
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2
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1
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2
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4
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1
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1
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644975
Beg
Human hepatic 3-hydroxy-3-meth ...
Homo sapiens
Biochem. Biophys. Res. Commun.
119
488-498
1984
-
-
-
-
-
-
-
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1
1
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1
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2
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1
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1
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2
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1
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1
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1
1
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1
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1
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2
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1
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1
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-
-
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-
-
286565
Ingebritsen
Regulation of liver hydroxymet ...
Rattus norvegicus, Rattus norvegicus Wistar
J. Biol. Chem.
256
1138-1144
1981
1
-
-
-
-
-
1
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2
1
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2
-
166
-
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1
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1
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4
-
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1
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1
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1
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2
1
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2
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1
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1
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4
-
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-
-
1
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-
-
-
-
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644971
Beg
Characterization and regulatio ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
76
4375-4379
1979
1
-
-
-
-
-
1
-
2
1
1
2
-
1
-
-
1
-
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1
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6
-
2
-
-
-
1
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1
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1
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1
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2
1
1
2
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1
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1
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-
6
-
2
-
-
-
1
-
-
1
-
-
-
-
-
-
644953
Ingebritsen
Reversible modulation of the a ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
81
1268-1277
1978
-
-
-
-
-
-
1
1
1
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1
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1
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-
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1
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2
-
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1
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1
1
1
1
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1
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1
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2
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