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Literature summary for 2.7.11.26 extracted from

  • Aoki, M.; Yokota, T.; Sugiura, I.; Sasaki, C.; Hasegawa, T.; Okumura, C.; Ishiguro, K.; Kohno, T.; Sugio, S.; Matsuzaki, T.
    Structural insight into nucleotide recognition in tau-protein kinase I/glycogen synthase kinase 3 beta (2004), Acta Crystallogr. Sect. D, 60, 439-446.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activation loop structure Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of GSK3beta in Escherichia coli in fusion with a Leu-Glu-His6-tag at the C-terminus Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant Leu-Glu-His6-tagged GSK3beta in complex with ATP or ATP analogue AMP-PNP, 10 mg/ml protein, 2 mM ATP or AMP-PNP, 12-14% w/v PEG 6000, 100 mM NaCl, 5 mM MgCl2, 10% v/v glycerol, in 100 mM HEPES-NaOH, pH 7.5, hanging drop vapor diffusion method, 4°C, several days, soaking in 0.1 mM ethylmercuric thiosalicylate at pH 7.5 for 1 h, cryoprotection by 30% w/v D-sorbitol, X-ray diffraction structure determination and analysis at 1.7-2.6 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP-PNP ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ binding site structure Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme participates in Alzheimer's disease ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Leu-Glu-His6-tagged GSK3beta from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + [tau-protein] = ADP + O-phospho-[tau-protein] activation loop structure, substrate binding structure, and catalytic site structure and mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [tau-protein]
-
Homo sapiens ADP + O-phospho-[tau-protein]
-
?
additional information the enzyme participates in Alzheimer's disease Homo sapiens ?
-
?
additional information substrate specificity of GSK3beta Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
glycogen synthase kinase 3beta
-
Homo sapiens
GSK3beta
-
Homo sapiens
More see also EC 2.7.11.1 Homo sapiens
tau-protein kinase I
-
Homo sapiens
TPK I
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP binding site structure Homo sapiens