Literature summary for 2.7.11.24 extracted from

Maisonneuve, P.; Caillet-Saguy, C.; Vaney, M.; Bibi-Zainab, E.; Sawyer, K.; Raynal, B.; Haouz, A.; Delepierre, M.; Lafon, M.; Cordier, F.; Wolff, N.
Molecular basis of the interaction of the human protein tyrosine phosphatase non-receptor type 4 (PTPN4) with the mitogen-activated protein kinase p38gamma (2016), J. Biol. Chem., 291, 16699-16708.

Search for more literature for 2.7.11.24

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	p38gamma is phosphorylated and activated by MAPK kinase MKK6	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
isolated PDZ domain of protein tyrosine phosphatase non-receptor type bound to the p38gamma C-terminus, i.e. Cyto8-RETEV and p38gamma-KETPL peptides, mixing of the protein ligand solution from 5 mg/ml PTPN4-PDZ domain and the appropriate peptide at a ratio of 1:2 and 1:4 for PTPN4-PDZCyto8-RETEV and PTPN4-PDZp38gamma-KETPL, respectively, in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM TCEP, sitting drop vapor diffusion method, mixing of 400 nl protein-ligand solution with 200 nl of precipitant solution containing 23% PEG 8000, 100 mM MES, pH 6.0, 200 mM calcium acetate, 0.143 mM ammonium sulfate or 1.2 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.1 M CAPS, pH 10.5, 0.2 M lithium sulfate, 0.67 M non-detergent sulfobetaine (NDSB) 201, 18°C, X-ray diffraction structure determination and analysis at 2.09-2.35 A resolution, modeling	Homo sapiens

Crystallization (Comment)

Organism

isolated PDZ domain of protein tyrosine phosphatase non-receptor type bound to the p38gamma C-terminus, i.e. Cyto8-RETEV and p38gamma-KETPL peptides, mixing of the protein ligand solution from 5 mg/ml PTPN4-PDZ domain and the appropriate peptide at a ratio of 1:2 and 1:4 for PTPN4-PDZCyto8-RETEV and PTPN4-PDZp38gamma-KETPL, respectively, in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM TCEP, sitting drop vapor diffusion method, mixing of 400 nl protein-ligand solution with 200 nl of precipitant solution containing 23% PEG 8000, 100 mM MES, pH 6.0, 200 mM calcium acetate, 0.143 mM ammonium sulfate or 1.2 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.1 M CAPS, pH 10.5, 0.2 M lithium sulfate, 0.67 M non-detergent sulfobetaine (NDSB) 201, 18°C, X-ray diffraction structure determination and analysis at 2.09-2.35 A resolution, modeling

Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	constants of dissociation and thermodynamic parameters of p38gamma binding with different PTPN4 constructs, overview	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P53778	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	p38gamma is phosphorylated and activated by MAPK kinase MKK6	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
neuroblastoma cell	-	Homo sapiens	-
SH-SY5Y cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
MAPK12	-	Homo sapiens
p38gamma	-	Homo sapiens

General Information

General Information	Comment	Organism
additional information	molecular basis of the interaction of the human protein tyrosine phosphatase non-receptor type 4 (PTPN4) with the mitogen-activated protein kinase p38gamma, the main contribution to the p38gamma-PTPN4 complex formation is the tight interaction between the C-terminus of p38gamma and the PDZ domain of PTPN4, overview. NMR titration study at pH 7.5, 25°C	Homo sapiens
physiological function	the p38gamma C-terminus is an efficient inducer of cell death after its intracellular delivery. Binding of the C-terminal sequence of p38gamma to PTPN4 abolishes the catalytic autoinhibition of PTPN4 and thus activates the phosphatase, which can efficiently dephosphorylate the activation loop of p38gamma. The p38gamma-PTPN4 interaction promotes cellular signaling, preventing cell death induction	Homo sapiens