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Literature summary for 2.7.11.24 extracted from

  • Xiao, Y.; Warner, L.R.; Latham, M.P.; Ahn, N.G.; Pardi, A.
    Structure-based assignment of Ile, Leu, and Val methyl groups in the active and inactive forms of the mitogen-activated protein kinase extracellular signal-regulated kinase 2 (2015), Biochemistry, 54, 4307-4319.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.24

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant 0P-ERK2 proteins in Escherichia coli strain BL21(DE3) Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis of ERK2, PDB ID 1ERK Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
I101A site-directed mutagenesis Rattus norvegicus
I124A site-directed mutagenesis Rattus norvegicus
I238A site-directed mutagenesis Rattus norvegicus
L102A/Q103A site-directed mutagenesis Rattus norvegicus
L105A site-directed mutagenesis Rattus norvegicus
L110A site-directed mutagenesis Rattus norvegicus
L113A site-directed mutagenesis Rattus norvegicus
L154A site-directed mutagenesis Rattus norvegicus
L155A site-directed mutagenesis Rattus norvegicus
L161A site-directed mutagenesis Rattus norvegicus
L198A site-directed mutagenesis Rattus norvegicus
L235A site-directed mutagenesis Rattus norvegicus
additional information wild-type and mutant binding analysis with nonphosphorylated and phosphorylated cofactor and derivative, i.e. ATP and AMP-PNP, overview Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P63086
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 0P-ERK2 wild-type and mutants from Escherichia coli strain BL21(DE3) Rattus norvegicus

Synonyms

Synonyms Comment Organism
ERK
-
 Rattus norvegicus
extracellular signal-regulated kinase 2
-
 Rattus norvegicus
MAP kinase
-
 Rattus norvegicus
MAPK
-
 Rattus norvegicus
MAPK1
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus
additional information nonactive ATP homologue AMP-PNP induces long-range effects in both active and inactive ERK2, and AMP-PNP shows slow kinetics for binding to ERK2 on the NMR chemical shift time scale. Wild-type and mutant binding analysis with nonphosphorylated and phosphorylated cofactor and derivative, i.e. ATP and AMP-PNP, overview Rattus norvegicus

General Information

General Information Comment Organism
additional information structure-based assignment of Ile, Leu, and Val methyl groups in the active and inactive forms of extracellular signal-regulated kinase 2, overview Rattus norvegicus
physiological function ERK2 is a critical component in the mitogen-activated protein kinase signal cascade, where it helps regulate many cellular processes including proliferation, differentiation, and survival Rattus norvegicus