Any feedback?
Literature summary for 2.7.11.23 extracted from
- A universal RNA polymerase II CTD cycle is orchestrated by complex interplays between kinase, phosphatase, and isomerase enzymes along genes (2012), Mol. Cell, 45, 158-170.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a bur1 knockout mutant bur1DELTA | Saccharomyces cerevisiae |
| additional information | generation of a ctk1 knockout mutant ctk1DELTA | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity of CTD kinase Bur1 is repressed in the presence of a functional CTD kinase Kin28 complex | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | profiling of the location of the RNAPII phosphorylated isoforms in wild-type cells and mutants for most CTD modifying enzymes. The CTD cycle is uniform across genes | Saccharomyces cerevisiae | - |
- |
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | - |
ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
| ATP + [DNA-directed RNA polymerase] | Saccharomyces cerevisiae | Ctk1 is the major serine 2 kinase in vivo | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P06242 | - |
- |
| Saccharomyces cerevisiae | P23293 | - |
- |
| Saccharomyces cerevisiae | Q03957 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + C-terminal domain of RNA polymerase II | the recombinant GST-tagged CTD substrate is hyperphosphorylated by Kin28 | Saccharomyces cerevisiae | ADP + phosphorylated C-terminal domain of RNA polymerase II | - |
? | |
| ATP + [DNA-directed RNA polymerase] | - |
Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
| ATP + [DNA-directed RNA polymerase] | Ctk1 is the major serine 2 kinase in vivo | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
| ATP + [DNA-directed RNA polymerase] | in vitro, Ctk1 complex can phosphorylate the CTD at all three serines, although with different efficiencies. Ctk1 phosphorylates Ser2 in vitro | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
| ATP + [DNA-directed RNA polymerase] | phosphorylation of Ser2, Ser5, and Ser7 in CTD | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
| ATP + [DNA-directed RNA polymerase] | phosphorylation of Ser5 and Ser7 in CTD, Bur1 phosphorylates Ser2 in vitro | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bur1 | - |
Saccharomyces cerevisiae |
| CTD kinase | - |
Saccharomyces cerevisiae |
| Ctk1 | - |
Saccharomyces cerevisiae |
| Kin28 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the universal RNA polymerase II CTD cycle is orchestrated by complex interplays between kinase, phosphatase, and isomerase enzymes along genes | Saccharomyces cerevisiae |
| physiological function | transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS involving a CTD kinase. The CTD cycle is uniform across genes. Proline isomerization is a key regulator of CTD dephosphorylation at the end of genes. Bur1 is a potent Ser5 and Ser7 kinase | Saccharomyces cerevisiae |
| physiological function | transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS involving a CTD kinase. The CTD cycle is uniform across genes. Proline isomerization is a key regulator of CTD dephosphorylation at the end of genes. Enzyme Ctk1 does not play any significant role in maintaining P-Ser5 and P-Ser7 levels in wild-type cells, the phosphorylation activity versus Ser5 and Ser7 is mainly in an opportunistic manner. Ctk1 is the major serine 2 kinase during transcription in vivo | Saccharomyces cerevisiae |
| physiological function | transcription by RNA polymerase II (RNAPII) is coupled to mRNA processing and chromatin modifications via the C-terminal domain (CTD) of its largest subunit, consisting of multiple repeats of the heptapeptide YSPTSPS involving a CTD kinase. The CTD cycle is uniform across genes. Proline isomerization is a key regulator of CTD dephosphorylation at the end of genes. Kin28 is the main CTD kinase. sThe kinase activity of Kin28 downregulates the activity of Bur1 against P-Ser5 and 7 along the coding region | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
