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Literature summary for 2.7.11.23 extracted from

  • Kapasi, A.J.; Spector, D.H.
    Inhibition of the cyclin-dependent kinases at the beginning of the human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinases cdk9 and cdk7 at the viral transcriptosome (2007), J. Virol., 82, 394-407.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
cyclin T1 is in a complex with cdk9 and forms P-TEFb Homo sapiens

Application

Application Comment Organism
medicine IE2 and multiple cellular factors needed for viral RNA synthesis accumulate within the first 8 h at the viral transcriptosome, functional cdk activity is required for the specific recruitment of cdk7 and cdk9 during this time interval Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cDNA copy of cdk9HA placed into a lentiviral vector where its transcription is driven by an EF1alpha promoter, overexpression in HFF cells Homo sapiens
overexpression of HA-tagged cdk9 in 293FT cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
roscovitine impairment in the localization of proteins to the immediate-early transcription sites during roscovitine treatment is specific to cdk9 and cdk7, decreases levels of cdk9 and cdk7 RNA, a corresponding reduction in cdk9 protein but only a modest decrease in cdk7 protein, a delay in the addition of roscovitine until 8 h p.i. does not inhibit cdk9 and cdk7 protein expression in human cytomegalovirus-infected cells, overexpression of cdk9 does not compensate for the effects of roscovitine on its localization in human cytomegalovirus-infected cells; inhibition of cdk7 expression during roscovitine treatment occurs at the RNA level; inhibition of cdk9 expression during roscovitine treatment occurs at the RNA level, overexpression of cdk9 does not compensate for the effects of roscovitine on its localization in HCMV-infected cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information localization of cdk7 and cdk9 to the viral transcriptosome of human cytomegalovirus Homo sapiens
-
-
additional information human cytomegalovirus, HCMV, infection in the presence of roscovitine impairs the localization of cdk7, but not of IE2-86, at viral transcriptosomes Homo sapiens
-
-
additional information human cytomegalovirus, HCMV, infection in the presence of roscovitine impairs the localization of cdk9, but not of IE2-86, at viral transcriptosomes Homo sapiens
-
-
nucleus
-
 Homo sapiens 5634
-
transcriptosome
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RNA polymerase II C-terminal subunit Homo sapiens i.e. RNAP II CTD, hypophosphorylated form of RNAP IIa is recruited to the preinitiation complex at the gene promoter by the general transcription factors. Initiation proceeds when the cdk7 complex phosphorylates the CTD at the serine 5 residues, resulting in a hyperphosphorylated RNAP II that recruits the RNA-capping enzymes, overview ADP + phosphoylated RNA polymerase II C-terminal subunit
-
 ?
additional information Homo sapiens cdk7 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview ?
-
 ?
additional information Homo sapiens cdk9 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P50613 cdk7
-
Homo sapiens P50750 cdk9
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast of foreskin Homo sapiens
-
additional information HFF cell Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA polymerase II both cdk7 and cdk9 are responsible for phosphorylation at the C-terminal domain Homo sapiens ?
-
 ?
ATP + RNA polymerase II C-terminal subunit i.e. RNAP II CTD, hypophosphorylated form of RNAP IIa is recruited to the preinitiation complex at the gene promoter by the general transcription factors. Initiation proceeds when the cdk7 complex phosphorylates the CTD at the serine 5 residues, resulting in a hyperphosphorylated RNAP II that recruits the RNA-capping enzymes, overview Homo sapiens ADP + phosphoylated RNA polymerase II C-terminal subunit
-
 ?
ATP + RNA polymerase II C-terminal subunit i.e. RNAP II CTD, phosphorylation at Ser5 Homo sapiens ADP + phosphoylated RNA polymerase II C-terminal subunit
-
 ?
additional information cdk7 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview Homo sapiens ?
-
 ?
additional information cdk9 of the host transcription machinery is recruited to human cytomegalovirus immediate-early transcription sites, inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the RNA polymerase II carboxyl-terminal domain kinase cdk7 at the viral transcriptosome, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cdk7
-
 Homo sapiens
CDK9
-
 Homo sapiens
RNA polymerase II carboxyl-terminal domain kinase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens